NEBNext® Fast DNA Library Prep Set for Ion Torrent 4

Catalog number: 4474177

Ion Torrent™  Related applications: Ion Torrent™ Next-Generation Sequencing

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The NEBNext® Fast DNA Library Prep Set for Ion Torrent 4 provides a fast, high yield protocol for production of libraries suitable for sequencing on the Ion Torrent™ platform. All required reagents are supplied for construction of libraries from inputs of 10 ng–1 µg DNA, in just over 2 hours with minimal hands-on time. This set is for use with DNA that has been sheared by physical methods. For enzyme-based DNA fragmentation, please see the NEBNext® Fast DNA Fragmentation & Library Prep Set for Ion Torrent 4, which includes fragmentation reagents.


• Fast (just over 2 hours), with minimal hands-on time

• High yield

• Simple protocol

• Streamlined workflow

• Compatible with a broad range of sample input (10 ng–1 µg*)

• Effective with input DNA of varying GC content

• Also available with optional enzyme-based fragmentation

• Multiplex compatible

• Stringent quality controls

• Validated on the Ion Torrent™ platform

*See "Multiplex Compatiblity" note below.

Multiplex Compatibility

The NEBNext® Fast DNA Library Prep sets are compatible with the Ion Xpress™ Barcode Adapters 1-16 Kit with a minimum recommended input of 100 ng DNA.

Two Sizes

Each set is available in 10 reaction and 50 reaction sizes. In addition to increased convenience for higher throughput users, the 50 reaction size provides reduced pricing per reaction.


Individual components undergo rigorous QC analysis to help ensure maximum quality and purity. In addition, every lot undergoes functional validation by preparation of a genomic DNA library that is sequenced on the Ion Torrent™ platform.


For Use With (Equipment): Ion PGM™ System, Ion OneTouch™ System
Library Type: Fragment Library
Workflow Step: Library Generation
Starting Material: DNA
Sequencing Type: Genome & DNA Sequencing
Product Size: 10 reactions
Template Compatibility: Pre-sheared DNA
Starting Material (Amount): 100 ng - 2 µg DNA
High Throughput Compatibility: Multiplexing, High Throughput-Compatible
Shipping Condition: Dry Ice

Contents & storage


1 tube of NEBNext® End Repair Enzyme Mix

1 tube of NEBNext® End Repair Reaction Buffer

1 tube of T4 DNA Ligase

1 tube of T4 DNA Ligase Buffer for Ion Torrent (10X)

1 tube of NEBNext® DNA Library Primers for Ion Torrent

1 tube of NEBNext® DNA Library Adaptors for Ion Torrent

1 tube of TE Buffer (0.1X)

1 tube of NEBNext® High-Fidelity 2X PCR Master Mix

1 tube of Bst 2.0 WarmStart™ DNA Polymerase

Storage: Store all reagents at -20°C.


Manuals & protocols

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