The Ion AmpliSeq Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 speciallyRead more
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Catalog number 4479790
Price (USD)/ 96 reactions
223.00
-
Add to cart
Price (USD)/ 96 reactions
223.00
Add to cart
Ion AmpliSeq™ Sample ID Panel
Catalog number4479790
Price (USD)/ 96 reactions
223.00
-
Add to cart
The Ion AmpliSeq Sample ID Panel is a versatile, cost effective, and easy-to-use human SNP genotyping panel comprising 9 specially designed primer pairs that can be added to the multiplex PCR reaction to generate a unique ID during post-sequencing analysis of research samples.
Designed to advance clinical research, the Ion AmpliSeq Sample ID Panel helps reveal sample misidentification for increased confidence during data analysis and reporting. The Ion AmpliSeq Sample ID Panel can be used in conjunction with any Ion AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.ampliseq.com).
Verify various sample combinations: • Tumor/normal paired samples • Samples from the same individual in longitudinal studies • Multi-tissue or multi-tumor samples from the same individual
Sample identification through SNP genetyping • Panel includes nine primer pairs targeting nine (female samples) or ten (male samples) validated SNPs • SNPs are unlinked and exhibit exceptional robustness and consistently high minor allele frequency across a diverse group of human populations • One primer pair targets the amelogenin gene and amplifies one target on X chromosome and a distinct target on Y chromosome, allowing simple and quick sample gender determination • First letter of Sample ID code provides instant gender determination for sample • Optimal discrimination power (∼1:5,000*) with minimal sequencing needed
Simple workflow—just one pipetting step • Spike 1 μL of the 20X Sample ID primer pool directly into any Ion AmpliSeq primer pool for co-amplification and downstream sequencing and data analysis • Enabled for use with any Ion Xpress barcode • Compatible with the Ion Reporter Software, a cloud-hosted software tool for automated variant analysis
Includes: 20X Ion AmpliSeq Sample ID Primer Pool
Required: • Torrent Suite Software (v3.0 or greater) that includes the Ion AmpliSeq Sample ID Panel plug-in • Ion AmpliSeq Library Kit 2.0 User Guide • Ion AmpliSeq ready-to-use panel or custom panel designed via the Ion AmpliSeq Designer (www.ampliseq.com)
1Pakstis AJ et al. SNPs for a Universal Individual Identification Panel. Hum Genet. 2010 Mar; 127(3):315-24. *4641 discrimination power calculations assumes no missing genotyping data
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Sample TypeDNA
Sequencing TypeGenome and DNA Sequencing
For Use With (Equipment)Ion PGM™ System, Ion Proton™ System, Ion GeneStudio™ S5 System
For Use With (Application)Sequencing
LibrariesTargeted Sequencing Library
Multiplex Capability9 amplicons
PrimerSample ID Primers
Product LineIon AmpliSeq™
Product TypeSample ID Panel
Quantity96 reactions
Shipping ConditionApproved for shipment at Room Temperature or on Dry Ice
SpeciesHuman
TechniqueAmplicon Sequencing
Workflow StepSample QC
Starting Material Amount10 ng gDNA
Unit Size96 reactions
Contents & Storage
1 tube 20X Ion AmpliSeq™ Sample ID Primer Pool Store at -30 °C to -10 °C