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EGF Recombinant Mouse Protein, hIgG1-Fc Tag

Catalog number: 50482M01H50

 Related applications: Protein Assays and Analysis

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Description

Epidermal growth factor (EGF), recombinant mouse protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of mouse EGF (P01132) (Asn 977-Arg 1029) fused to the Fc region of human IgG1 at the N-terminus.

Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 0.2-1 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: AI790464
Gene Symbol: EGF
Molecular Weight: 34.5 kDa
Product Size: 50 µg
Protein Family: Growth Factors & Receptors
Protein Form: Full Length, Recombinant
Protein Subtype: EGF (Epidermal Growth Factor)
Protein Tag or Fusion: IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Cell Cycle & Proliferation, Other Research
Species: Mouse
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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