Axiom Trout Genotyping Array (384HT format) was designed under the Affymetrix Expert Design Program in collaboration with the National Center for Cool and Cold Water Aquaculture, USDA-ARS, USA, and AquaGen, Trondheim, Norway. The array includes 57,501 markers and is available in both 96 format and 384 format.
Axiom Trout Genotyping Array offers a standard high-throughput, cost-effective, and robust genotyping technology to conduct genome-wide association studies (GWAS), to study genetic architecture, perform marker-trait association, and to increase accuracy of breeding programs. The high marker density on the array ensures a broad coverage of the trout genome to provide representation of all regions in the genome.
Features of Axiom Trout Genotyping Array • Comprehensive content: The array includes 57,501 markers spaced across the genome as follows: - 17,000 markers that are unique to SNPs discovered in a previous USDA study1 - 20,000 markers unique to an outbred Norwegian commercial population - Amino acid shifting SNPs - SNPs preferentially located within a gene and with minor allele frequency (MAF) >0.2 - Y chromosome-specific SNPs near the sdY gene (male-specific in rainbow trout)2
Applications of Axiom Trout Genotyping Array • Complex trait research and molecular breeding - GWAS and quantitative trait locus (QTL) mapping - Identification of economically important traits - Improved accuracy in aquaculture breeding programs through genomic selection
• Population and evolutionary genetics - Development of new breeding populations - Differentiation between fish of different origins - Gender determination via sdY chromosome-specific probes - Differentiation of farmed and wild populations