Thermo Scientific™

Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 30 mL

Catalog number: 66130
Thermo Scientific™

Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 30 mL

Catalog number: 66130
Catalog Number
Unit Size
6 cassettes
Price (USD)
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
661306 cassettes
Your Price:
Product Overview
Citations & References
Additional Information
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (3.5K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 3,500 daltons.
The 30 mL size is convenient for many large-scale applications

Features of Slide-A-Lyzer Dialysis Cassettes:

Easy to use—no knots or clamps are needed; just inject sample into cassette and begin dialysis
Fast dialysis—flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
High recovery—rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
Four cassette sizes—select the cassette that best suits your sample volume
Color-coded—orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
Gamma-irradiated option—gamma-irradiated cassettes are available for applications requiring conditions that minimize the risk of sample contamination

10K Dialysis Membrane Specifications:
Composition: Regenerated cellulose synthesized by the Viscose method
Thickness: 0.9 to 1.2mil (22.5 to 30 µM)
Hydration Required Before Use: 30 seconds for small-volume samples
Glycerol Content: 21%
Sulfur Content: 0.05%
Heavy Metals Content: Trace

Slide-A-Lyzer Dialysis cassettes are available in four different sizes to accommodate sample volumes between 0.1 and 30 mL. These devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.

Gamma-irradiated Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications requiring conditions that minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth. However, a confirmation of sterility is not provided for individual lots, as no further sterility testing is conducted post irradiation.

Slide-A-Lyzer Dialysis Cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers.

More Product Data
Separation characteristics of dialysis membranes

Related Products
Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 0.5 mL
Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 3 mL
Slide-A-Lyzer™ Dialysis Cassettes, 3.5K MWCO, 12 mL
For Research Use Only. Not for use in diagnostic procedures.


Slide-A-Lyzer Dialysis Cassettes, 3.5K MWCO, 30mL
Product Line
Capacity (Metric)
30 mL
Cut Off or Exclusion Limit
3.5 kDa
Cellulose Membrane, Dialysis
Purification Target
Buffer Exchange, Protein
6 Cassette

Contents & Storage

Store at room temperature


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Frequently asked questions (FAQs)

Citations & References