Contains Sodium azide and formaldehyde

eBioscience™ Human Regulatory T Cell Staining Kit

Catalog number:  88-8999-40

Related applications:

Flow Cytometry

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This Human Regulatory T cell Staining Kit contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa glycoprotein found on the surface of the majority of thymocytes, a subset of mature T cells (T helper cells), and at lower levels on monocytes. The BC96 monoclonal antibody reacts with human CD25 (also known as interleukin-2 receptor alpha, IL-2R alpha), a 55 kDa surface protein expressed by early progenitors of T cells and B cells, by mature, activated T cells and B cells, and at constitutively high levels on regulatory T cells. The PCH101 monoclonal antibody reacts with the amino terminus of human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors. It was identified as the gene responsible for the X-linked lymphoproliferative disease observed in scurfy (sf) mice and in the human disorder, X-linked autoimmunity-allergic dysregulation syndrome (XLAAD). Constitutive expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.


Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Product Line: eBioscience™
Quantity: 1 kit
Storage Requirements: 2° to 8°C. Light sensitive material. Use within 6 months of opening or by date indicated on the bottle.
For Use With (Application): Intracellular Staining Followed by Flow Cytometric Analysis