Thermo Scientific™

Micrococcal Nuclease Solution (≥100 U/µL)

Catalog number: 88216
Thermo Scientific™

Micrococcal Nuclease Solution (≥100 U/µL)

Catalog number: 88216
Catalog Number
88216
Unit Size
150 µL
Price (USD)
Price: 167.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
88216150 µL
Price: 167.00
Your Price:
-
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Thermo Scientific Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

Features of Micrococcal Nuclease (MNase):

• Enzyme digests nucleic acids (DNA and RNA)
• Effective for double-stranded, single-stranded, circular and linear nucleic acids
• Active in neutral to alkaline buffer conditions containing divalent calcium ions
• Supplied at ≥100 units/µL in 10 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM EDTA, 50% glycerol

This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus. Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA. This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids. The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions. Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for digestion of RNA and DNA substrates. Micrococcal Nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments.

Properties of Micrococcal Nuclease (MNase):
• Product is a liquid at -20°C. Keep enzyme on ice during use.
• Micrococcal nuclease is dependent on Ca++ for activity. Avoid calcium chelators, such as EGTA, in reaction buffers.
• Enzyme is active at pH 7 to 10 with a salt concentration less than 100 mM. Optimal enzyme activity occurs at 37°C; however, the enzyme is active at room temperature. Monovalent metal ions, such as Na+ and K+, will decrease activity. EGTA or heating to 65°C for 10 minutes will inactivate the enzyme.

Generalized Protocol for use of Micrococcal Nuclease (MNase):
• Dilute sample in 50 mM Tris-HCl pH 8.0, 5 mM CaCl2
• If sample only contains nucleotides (i.e., no protein), add BSA at a final concentration of 0.1 mg/mL.
• Add micrococcal nuclease and incubate reaction at 37°C until the nucleotides are degraded.
• Optional: Stop reaction by adding EGTA, pH 8.0 to a final concentration of 20 mM.

Specifications of Micrococcal Nuclease (Part No. 88216):
• Visual: Clear, colorless liquid free of insoluble material.
• Units: ≥100 units/µL
• Additional Information: 1 unit is defined as the amount of enzyme required to produce an increase in absorbance at 260nm of 0.001/min/mL at 25°C of highly polymerized DNA.

Related Products
Pierce™ Universal Nuclease for Cell Lysis
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
150 µL
Form
Liquid
Reagent Type
Enzyme for Cell Lysis
Product Type
Micrococcal Nuclease Solution

Contents & Storage

Upon receipt store at -20°C. Product is shipped with dry ice.