Thermo Scientific™

Pierce™ S-Nitrosylation Western Blot Kit and HENS Buffer

Catalog number: 90105
Thermo Scientific™

Pierce™ S-Nitrosylation Western Blot Kit and HENS Buffer

Catalog number: 90105
The Pierce S-Nitrosylation Western Blot Kit provides cell lysis/reaction buffer, sulfhydryl-reactive blocking agent, reducing agent, labeling agent, and anti-TMT antibody to label 40 samples (100 μg each). As with traditional S-nitrosylation switch assays, unmodified cysteines are blocked by a sulfhydryl-reactive compound. S-nitrosylated cysteines are selectively reduced with ascorbate in HENS Buffer for labeling with iodoTMTzero reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Detection of the TMT reagent-modified proteins is facilitated using an anti-TMT antibody.
 
Catalog Number
90105
Unit Size
40 reactions
Description
Pierce™ S-Nitrosylation Western Blot Kit
Price (USD)
Price: 499.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionPierce™ S-Nitrosylation Western Blot Kit
Detection MethodS-Nitrosylation
Product LinePierce™
Quantity40 Reactions
ReactivityAll species
Unit Size40 reactions
Catalog Number
Unit Size
100 mL
Description
HENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit
Price (USD)
Price: 124.00
Your price:
Availability
-
Full specifications
Quantity
DescriptionHENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit
Detection MethodS-Nitrosylation
Product LinePierce™
Quantity100 mL
ReactivityAll species
Unit Size100 mL
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)AvailabilityQuantity
90105Full specifications
40 reactionsPierce™ S-Nitrosylation Western Blot Kit
Price: 499.00
Your price:
-
DescriptionPierce™ S-Nitrosylation Western Blot Kit
Detection MethodS-Nitrosylation
Product LinePierce™
Quantity40 Reactions
ReactivityAll species
Unit Size40 reactions
90106Full specifications
100 mLHENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit
Price: 124.00
Your price:
-
DescriptionHENS Buffer for Pierce™ S-Nitrosylation Western Blot Kit
Detection MethodS-Nitrosylation
Product LinePierce™
Quantity100 mL
ReactivityAll species
Unit Size100 mL
Showing 2 of 2
S-nitrosylation is a post-translational modification that regulates numerous processes, including cellular proliferation, apoptosis, smooth muscle relaxation, neurotransmitter release, and differentiation. During S-nitrosylation, nitric oxide radicals react with cysteine thiols to produce an S-NO adduct that alters protein activity.

Pierce S-Nitrosylation Western Blot Kit
The Pierce S-Nitrosylation Western Blot Kit provides the necessary reagents to perform a modified version of the traditional S-nitrosylation switch assay, including a cysteine-blocking reagent, reducing agent, reaction buffer, iodoTMT labeling reagent, and anti-TMT antibody. Labeling is accomplished using the non-biological iodoTMT reagent instead of HPDP-biotin, reducing background signal during western blot detection. In addition to detection of S-nitrosylated proteins by western blot, an immobilized anti-TMT resin can be used to selectively enrich S-nitrosylated proteins/peptides labeled with iodoTMT reagents. This workflow allows for S-nitrosylation site mapping and multiplexed quantitation using mass spectrometry.

Features of Pierce S-Nitrosylation Western Blot Kit include:
Specific—optimized labeling reactions switch S-nitrosocysteine post translational modifications with iodoTMT reagent for easy detection by western blot using anti-TMT antibody
Better signal-to-noise—uses a non-biological iodoTMT reagent for labeling instead of HPDP-biotin, resulting in less background during western blot detection
Workflow-compatible—labeling method is compatible with alternative methods of analysis, including enrichment via anti-TMT resin and multiplex quantitation by mass spectrometry
Convenient—all needed reagents included one kit

HENS Buffer
HENS Buffer consists of 100 mM HEPES (pH 7.8), 1 mM EDTA, 0.1 mM Neocuproine, and 1% SDS. It is a cell lysis buffer intended for use with the Pierce S-Nitrosylation Western Blot Kit, which enables sensitive detection of protein S-nitrosocysteine post-translational modifications in a complete, easy-to-use kit.

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Upon receipt store kit at –20°C, or store individual components as indicated above. Kit is shipped with an ice pack.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text