Streamline your causal variant studies by enriching genome-wide association studies (GWAS) datasets with an extensive set of novel, rare, putative functional variants. The content was discovered in 16 major exome sequencing projects spanning a total of 12,000 samples.
With the Axiom Exome 319 Array Plate, you get: • Comprehensive content for highest discovery power - More than 300,000 coding SNPs including non-synonymous and synonymous SNPs as well as variants in splice and stop codons. - Approximately 30,000 single-base and complex indels from the draft Phase 1 1000 Genomes Project exome calls. • Informative markers to enable integrated data analysis - AIMs: Ancestry informative markers to measure Latino and African American ancestry. All markers have low linkage disequilibrium (LD) and span all autosomes and chromosome X. - >GWAS tags: Includes over 5,000 SNPs reported in the NHGRI Catalog of Published Associations (August 2011) and additional unpublished hits from scientific collaborations. - High value: Contains other markers of interest, including DNA fingerprinting SNPs, human leukocyte antigen (HLA) genes, chromosome Y, mitochondrial DNA, and microRNA sequences. • Customization to include your candidate genes - Include an additional 100,000 SNPs from your own candidate genes to meet your research needs. Choose from the Axiom Genomic Database of 11M genotype-tested markers, or include variants of your own choosing. • Proven performance - The panel has been fully genotype-tested against stringent QC criteria to ensure high-quality data. - Every marker has been tested against approximately 1,200 samples to ensure high sample pass, call rate, and reproducibility.
Axiom Exome Genotyping Array Plates include: - Axiom 2.0 Reagent Kit for automated or manual processing of up to 96 samples per array plate. - A fully automated workflow with validated robotics methods for target preparation and array processing, based on the Beckman Biomek™ FXP Target Prep Express System and the GeneTitan™ MC Instrument. - A manual target preparation protocol for lower-throughput users. - Axiom Analysis Suite for automated allele calling and easier quality assessment of called genotypes.