Citations et références (13)

Gibco™

StemPro™ MSC SFM XenoFree

Le milieu GIBCO® StemPro® MSC SFM XenoFree a été développé pour la croissance et l’expansion de cellules souches mésenchymateuses (CSM)Afficher plus

Référence	Quantité
A1067501	1 kit

Référence A1067501

Prix (EUR)

592,00

Quantité:

1 kit

Prix (EUR)

592,00

Le milieu GIBCO® StemPro® MSC SFM XenoFree a été développé pour la croissance et l’expansion de cellules souches mésenchymateuses (CSM) et de cellules souches issues du tissu adipeux (CSTA) humaines dans des conditions complètement exemptes de sérum et de xéno-contaminants. L’utilisation du milieu StemPro® MSC SFM XenoFree permet l’expansion des CSM ou CSTA humaines pour de nombreux passages tout en maintenant leur phénotype multipotentiel (c’est-à-dire leur capacité à se différencier en lignées ostéogène, chondrogène et adipogène). Le milieu StemPro® MSC SFM XenoFree est fabriqué conformément aux BPFa, ce qui garantit la traçabilité et la fiabilité de la fabrication.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Type de celluleCellules souches mésenchymateuses

Environnement culturelCO2

Antibiotiques inclusPas d’antibiotiques

Gamme de produitsStemPro

Type de produitMilieux sans sérum de cellules souches (SFM)

Pureté ou qualitéQualifié cellule MS

Quantité1 kit

EspècesHumaine

ClassificationSans xéno-contaminants

Culture TypeCulture de cellules adhérentes

FormeLiquide

Serum LevelSans sérum

Sans additifSans glutamine, Sans rouge de phénol

Unit SizeEach

Contenu et stockage

Chaque kit contient 1 milieu de baseStemPro™ MSC SFM de 500 ml et 1 supplément sans xéno-contaminants StemPro™ MSC SFM de 5 ml. Le milieu basal SFM MSC StemPro™ est stocké entre 2 à 8°C et est stable pendant 12 mois. Le supplément SFM MSC StemPro™ sans xéno-contaminants est stocké congelé entre -5 à -20°C et est stable pendant 12 mois.

Foire aux questions (FAQ)

What is the concentration of sodium bicarbonate in StemPro MSC SFM XenoFree?

StemPro MSC SFM XenoFree contains 2.2 g/L of sodium bicarbonate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations et références (13)

Citations et références

Abstract

Exosomes Produced from 3D Cultures of MSCs by Tangential Flow Filtration Show Higher Yield and Improved Activity.

Authors:Haraszti RA,Miller R,Stoppato M,Sere YY,Coles A,Didiot MC,Wollacott R,Sapp E,Dubuke ML,Li X,Shaffer SA,DiFiglia M,Wang Y,Aronin N,Khvorova A

Journal:Molecular therapy : the journal of the American Society of Gene Therapy

PubMed ID:30341012

Exosomes can deliver therapeutic RNAs to neurons. The composition and the safety profile of exosomes depend on the type of the exosome-producing cell. Mesenchymal stem cells are considered to be an attractive cell type for therapeutic exosome production. However, scalable methods to isolate and manufacture exosomes from mesenchymal stem cells ... More

Diverse impact of xeno-free conditions on biological and regenerative properties of hUC-MSCs and their extracellular vesicles.

Authors:Bobis-Wozowicz S,Kmiotek K,Kania K,Karnas E,Labedz-Maslowska A,Sekula M,Kedracka-Krok S,Kolcz J,Boruczkowski D,Madeja Z,Zuba-Surma EK

Journal:Journal of molecular medicine (Berlin, Germany)

PubMed ID:27638341

ABSTRACT: Growing evidence indicates that intracellular signaling mediated by extracellular vesicles (EVs) released by stem cells plays a considerable role in triggering the regenerative program upon transplantation. EVs from umbilical cord mesenchymal stem cells (UC-MSC-EVs) have been shown to enhance tissue repair in animal models. However, translating such results into ... More

Toward a clinical-grade expansion of mesenchymal stem cells from human sources: a microcarrier-based culture system under xeno-free conditions.

Authors:Santos Fd,Andrade PZ,Abecasis MM,Gimble JM,Chase LG,Campbell AM,Boucher S,Vemuri MC,Silva CL,Cabral JM

Journal:Tissue engineering. Part C, Methods

PubMed ID:21895491

The immunomodulatory properties of mesenchymal stem cells (MSCs) make them attractive therapeutic agents for a wide range of diseases. However, the highly demanding cell doses used in MSC clinical trials (up to millions of cells/kg patient) currently require labor intensive methods and incur high reagent costs. Moreover, the use of ... More

A xeno-free microcarrier-based stirred culture system for the scalable expansion of human mesenchymal stem/stromal cells isolated from bone marrow and adipose tissue.

Authors:Carmelo JG, Fernandes-Platzgummer AM, Diogo MM, Lobato da Silva C, Cabral JM,

Journal:

PubMed ID:26136376

'Human mesenchymal stem/stromal cells (MSC) are promising candidates for cell-based therapies and the development of microcarrier-based cultures in scalable bioreactors with well-defined xenogeneic-free components represent important milestones towards the clinical-scale production of these cells. In this work, we optimized our previously developed xeno-free microcarrier-based system for the scalable expansion of ... More

Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.

Authors:Oikonomopoulos A, van Deen WK, Manansala AR, Lacey PN, Tomakili TA, Ziman A, Hommes DW,

Journal:

PubMed ID:26564250

'Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in ... More

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