StemPro MSC SFM XenoFree is a serum-free medium for the growth and expansion of mesenchymal stem cell (MSCs) derived from a variety of tissue sources. MSCs can be expanded for multiple passages while maintaining trilineage mesoderm differentiation potential. This medium supports scale-up 3D cell culture using microcarriers and extracellular vesicle production under serum-free and xeno-free conditions.