Invitrogen™

Human Dopaminergic Neuron Immunocytochemistry Kit

Catalog number:  A29515

 Related applications:

Cellular Imaging

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Description

The Human Dopaminergic Neuron Immunocytochemistry Kit enables optimal image-based analysis of three key markers of neural differentiation: OTX2, FoxA2, and tyrosine hydroxylase (TH). This high-performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment.

The Human Dopaminergic Neuron Immunocytochemistry Kit enables you to:
• Confirm expression of key cellular markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents

Confirm expression of key cellular markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of floor plate progenitors and dopaminergic neurons. OTX2 and FoxA2 are critical nuclear markers while TH is an important cytoplasmic marker.

Get more information per sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OTX2 and FoxA2, and FoxA2 and TH) along with nuclear DNA staining to help save time and precious sample.

Generate beautiful multiplexed images
Powered by the superior performance of bright, photostable Alexa Fluor™ dyes, this kit enables the acquisition of beautiful multiplexed images of key neural differentiation marker expression using commonly available blue, green, and orange/red filter sets.

Minimize unnecessary wash steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols, helping to save time and decrease the likelihood of cell loss during the staining protocol. The only washes required are after incubation with primary and secondary antibodies.

Process samples with confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 40 reactions
Shipping Condition: Dry Ice

Contents & storage

• 10 µL of Goat Anti-Human OTX2 (2000X)
• 10 µL of Mouse Anti-Human FoxA2 (1000X)
• 10 µL of Rabbit Anti-Human Tyrosine Hydroxylase (1000X)
• 20 µL of Alexa Fluor 488 Donkey Anti-Goat IgG (250X)
• 20 µL of Alexa Fluor 488 Donkey Anti-Rabbit IgG (250X)
• 20 µL of Alexa Fluor 555 Donkey Anti-Mouse (250X)
• 20 µL of Alexa Fluor 594 Donkey Anti-Mouse (250X)
• 1 vial of NucBlue Fixed Cell ReadyProbes Reagent (1X)
• 10 mL of Fixative Solution (1X)
• 10 mL of Permeabilization Solution (1X)
• 20 mL of Blocking Solution (1X)
• 20 mL of Wash Buffer (10X)

Store at -20 to 4°C.

Documents

Manuals & protocols