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인용 및 참조 문헌 (35)
Invitrogen™
Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer
Tetrafluorophenyl (TFP) ester는 전형적으로 생체분자의 일차 아민에 fluorophores 또는 hapten을 부착하는데 사용되는 succinimidyl ester (SE 또는 NHS-ester) 화학물질보다 개선된 형태입니다.자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| A30005 | 1 mg |
카탈로그 번호 A30005
제품 가격(KRW)
558,000
온라인 행사
Ends: 31-Dec-2025
697,000할인액 139,000 (20%)
Each
수량:
1 mg
제품 가격(KRW)
558,000
온라인 행사
Ends: 31-Dec-2025
697,000할인액 139,000 (20%)
Each
Tetrafluorophenyl (TFP) ester는 전형적으로 생체분자의 일차 아민에 fluorophores 또는 hapten을 부착하는데 사용되는 succinimidyl ester (SE 또는 NHS-ester) 화학물질보다 개선된 형태입니다. 두 반응성 화학물질 모두 dye 또는 hapten과 관심대상 성분 사이에서 동일하게 강력한 아민 결합을 형성시키지만, TFP ester가 접합 반응 중의 자연적 가수분해에 대해 덜 민감합니다. Alexa Fluor TFP ester는 일반적으로 반응에 사용되는 기본 pH에서 수 시간 동안 안정하며 succinimidyl ester보다 훨씬 오래 지속됩니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 반응성Amine
라벨 또는 염료Alexa Fluor™ 488
제품 유형Carboxylic Acid Tetrafluorophenyl Ester
수량1 mg
반응성 부분Active Ester, TFP Ester
배송 조건Room Temperature
라벨 유형Alexa Fluor
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
Store in freezer (-5 to -30°C) and protect from light.
자주 묻는 질문(FAQ)
I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?
I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?
인용 및 참조 문헌 (35)
인용 및 참조 문헌
Abstract
Identification, cloning, and characterization of two N-acetylgalactosamine-binding lectins from the albumen gland of Helix pomatia.
Journal:J Biol Chem
PubMed ID:21372134
Helix pomatia agglutinin (HPA), the lectin from the albumen gland of the Roman snail, has been used in histochemical studies relating glycosylation changes to the metastatic potential of solid tumors. To facilitate the use of HPA in a clinical (diagnostic) setting, detailed analysis of the lectin, including cloning and recombinant
Heterogeneous nuclear ribonucleoprotein (hnRNP) E1 binds to hnRNP A2 and inhibits translation of A2 response element mRNAs.
Journal:Mol Biol Cell
PubMed ID:16775011
'Heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a trans-acting RNA-binding protein that mediates trafficking of RNAs containing the cis-acting A2 response element (A2RE). Previous work has shown that A2RE RNAs are transported to myelin in oligodendrocytes and to dendrites in neurons. hnRNP E1 is an RNA-binding protein that regulates translation of
Photolithographic patterning of polyethylene glycol hydrogels.
Journal:Biomaterials
PubMed ID:16375965
'A simple, inexpensive photolithographic method for surface patterning deformable, solvated substrates is demonstrated using photoactive poly(ethylene glycol) (PEG)-diacrylate hydrogels as model substrates. Photolithographic masks were prepared by printing the desired patterns onto transparencies using a laser jet printer. Precursor solutions containing monoacryloyl-PEG-peptide and photoinitiator were layered onto hydrogel surfaces. The
Physical interactions of the peroxisomal targeting signal 1 receptor pex5p, studied by fluorescence correlation spectroscopy.
Journal:J Biol Chem
PubMed ID:12930827
'We have studied Hansenula polymorpha Pex5p and Pex8p using fluorescence correlation spectroscopy (FCS). Pex5p is the Peroxisomal Targeting Signal 1 (PTS1) receptor and Pex8p is an intraperoxisomal protein. Both proteins are essential for PTS1 protein import and have been shown to physically interact. We used FCS to analyze the molecular
Pathway of actin filament branch formation by Arp2/3 complex revealed by single-molecule imaging.
Journal:Proc Natl Acad Sci U S A
PubMed ID:23292935
'Actin filament nucleation by actin-related protein (Arp) 2/3 complex is a critical process in cell motility and endocytosis, yet key aspects of its mechanism are unknown due to a lack of real-time observations of Arp2/3 complex through the nucleation process. Triggered by the verprolin homology, central, and acidic (VCA) region