Invitrogen™

Alexa Fluor™ 488 5-TFP (Alexa Fluor™ 488 Carboxylic Acid, 2,3,5,6-Tetrafluorophenyl Ester), 5-isomer

Catalog number:  A30005

Related applications:

Protein Labeling & Crosslinking

| Labeling Chemistry

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Description

Tetrafluorophenyl (TFP) esters are an improvement over the succinimidyl ester (SE or NHS-ester) chemistry typically used to attach fluorophores or haptens to the primary amines of biomolecules. Both reactive chemistries produce the same strong amide bond between the dye or hapten and the compound of interest, but TFP esters are less susceptible to spontaneous hydrolysis during conjugation reactions. Alexa Fluor TFP esters are stable for several hours at the basic pH typically used for reactions – far outlasting succinimidyl esters.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Chemical Reactivity: Amine
Shipping Condition: Room Temperature
Label Type: Alexa Fluor Dyes
Label or Dye: Alexa Fluor™ 488
Product Line: Alexa Fluor™
Reactive Moiety: Active Ester, TFP Ester

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.