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Invitrogen™

Alexa Fluor™ Plus 405 Phalloidin

Catalog number:  A30104

 Related applications:

Cell Structure

| Cellular Imaging

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Description

Alexa Fluor Plus 405 Phalloidin is a high-affinity filamentous actin (F-actin) probe (phalloidin) conjugated to our bright, photostable, violet-fluorescent Alexa Fluor 405 dye.

• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/emission: 405/450 nm. Compatible with standard DAPI filter set or 405/violet excitation laser.
• Superior to antibody staining
• Optimal for fixed and permeabilized samples

Get superior results in your actin staining studies
Phalloidin is a bi-cyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides ""death cap"" mushroom and commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor Plus 405 provides violet fluorescence of unparalleled brightness and photostability.

Use in multiple applications
Alexa Fluor Plus 405 Phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Its staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Alexa Fluor™ Plus 405
Sub-Cellular Localization: Actin
Excitation⁄Emission (nm): 405/450
Product Line: Alexa Fluor™ Plus
Product Size: 1 vial
Shipping Condition: Qualified for Ambient or Wet Ice

Contents & storage

1 vial sufficient for 300 units/slides

Store at ≤-20°C after receiving
Desiccate, and protect from light
When stored as directed, product is stable for at least 1 year.

Documents

Manuals & protocols