Invitrogen™

Poly(A) Tailing Kit

Catalog number: AM1350
Invitrogen™

Poly(A) Tailing Kit

Catalog number: AM1350
Catalog Number
AM1350
Unit Size
25 reactions
Price (USD)
248.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM135025 reactions
248.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency. Sufficient reagents are included for 25 reactions.

• Adds a poly(A) tail of at least 150 nucleotides in length to the 3' termini of RNA
• Enhances translational efficiency in vivo
• Reaction can be adjusted to control for tail length
• Optimized for use with RNA transcripts synthesized using the mMESSAGE mMACHINE™ Kit

The Poly(A) Tailing Kit uses E. coli Poly(A) Polymerase I (E-PAP) to polyadenylate the 3'-termini of in vitro transcribed RNA. Polyadenylation plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. In the Poly(A) Tailing Kit, the E-PAP reaction has been optimized so that mRNAs are efficiently tailed with at least 150 adenines. The additional adenine residues confer stability to the mRNA and may increase translational efficiency of in vitro synthesized capped RNA in microinjection and transfection experiments (see Figure).

Accessory Products:
The Poly(A) Tailing Kit is optimized for use with Ambion's mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kit. mMESSAGE mMACHINE™ Kits are available with T7 (SKU #AM1334), T3 (SKU #AM1338), or SP6 (SKU #AM1330) polymerases.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Includes
Kit
Shipping Condition
Dry Ice
Final Product Type
In Vitro Transcribed RNA (Polyadenylated)
For Use With (Application)
RNA Transcription
Kit Type
Tailing Kit
No. of Reactions
25 Reactions
Product Line
Ambion™
Starting Material
RNA (In Vitro Transcribed)

Contents & Storage

E-PAP, 5X E-PAP Buffer, ATP Solution, MnCl2, and Control DNA Template should be stored at –20°C. Nuclease-free Water may be stored at any temperature.

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