The Ambion™ RNAqueous™-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous™-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation Technology
Vacuum Filter Plate (Glass Fiber Filter)
Elution Volume
105 μL
Sample Type
Cells, Tissue
Final Product Type
Total RNA
High-throughput Compatibility
High-throughput Compatible
For Use With (Equipment)
MultiPROBE™ II HT, Biomek™ 2000, Liquid Handling Systems
• 4 Filter Plates; store at room temperature • 4 Collection Plates with lids; room temperature • 4 Culture Plates; room temperature • 1 Heat Plate with Lid; room temperature • 100 mL Nuclease-free Water; room temperature • 100 mL Elution Solution; room temperature • 100 mL Lysis/Binding Solution; 4°C • 100 mL Rebinding Mix; 4°C • 2 x 175 mL Wash Solution (concentrated); 4°C • 1.25 mL DNase 1; -20°C • 12 mL DNase 1 Buffer; -20°C