RNAqueous® Total RNA Isolation Kit

Catalog number: AM1912

Ambion™  Related applications: RNA Extraction

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Description

The RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or from 107–108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous® Kit provides:

• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples

How it works
The RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria, Yeast, Cells, Plant Samples, Viral Samples, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, cDNA Library Construction, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays
Product Size: 50 preps
Final Product: Total RNA
Isolation Technology: Spin Column (Glass Fiber Filter)
Shipping Condition: Room Temperature

Contents & storage

• 6 ml Formaldehyde Load Dye (-20°C)
• 60 ml Lysis/Binding Solution (4°C)
• 60 ml Water for 64% Ethanol (4°C)
• 4 ml Lithium Chloride Precipitation Solution (4°C)
• 40 ml Wash Solution 1 (4°C)
• 80 ml Wash Solution 2/3 Concentrate (4°C)
• 50 Filter Cartridges (room temperature)
• 100 Collection Tubes (room temperature)
• 10 ml Elution Solution (-20°C, 4°C, or room temperature)

Documents

Manuals & protocols

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