RNAqueous™ Total RNA Isolation Kit

RNAs less than 200 nt are lost using the columns in the RNAqeous family of kits. These kits are not suitable for isolation of 5S rRNA or tRNA. The RNAqueous-Micro Total RNA Isolation Kit does, however, offer a modified protocol to allow isolation of miRNA from the sample.

To prevent filter clogging with viscous samples or samples containing a large amount of tissue, perform a clarifying centrifugatoin at 10,000-15,000 x g and remove to a fresh tube prior to adding ethanol to the lysis/binding solution.

RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).