Invitrogen™

      RNAqueous™ Total RNA Isolation Kit

      RNAqueous™ Total RNA Isolation Kit

      Catalog number:  AM1912

       Related applications: RNA Extraction

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      Description

      The RNAqueous® Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or from 107–108 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous® Kit provides:

      • Convenient syringe-based filtration
      • Compatibility with vacuum manifolds when processing multiple samples

      How it works
      The RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Sample Type (General): Bacteria, Cells, Plant Samples, Tissue, Viral Samples, Yeast
      High Throughput Compatibility: Not High Throughput-Compatible (Manual)
      Downstream Application: Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
      Final Product: Total RNA
      Isolation Technology: Spin Column (Glass Fiber Filter)
      Product Line: Ambion™, RNAqueous®
      Product Size: 50 preps
      Shipping Condition: Room Temperature

      Contents & storage

      • 6 ml Formaldehyde Load Dye (-20°C)
      • 60 ml Lysis/Binding Solution (4°C)
      • 60 ml Water for 64% Ethanol (4°C)
      • 4 ml Lithium Chloride Precipitation Solution (4°C)
      • 40 ml Wash Solution 1 (4°C)
      • 80 ml Wash Solution 2/3 Concentrate (4°C)
      • 50 Filter Cartridges (room temperature)
      • 100 Collection Tubes (room temperature)
      • 10 ml Elution Solution (-20°C, 4°C, or room temperature)

      Documents

      Manuals & protocols