Invitrogen™
RNAqueous™ Total RNA Isolation Kit
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Description
The RNAqueous® Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous® Kit provides:• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous® Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Sample Type (General): | Bacteria, Cells, Plant Samples, Tissue, Viral Samples, Yeast |
|---|---|
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Downstream Application: | Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction |
| Final Product: | Total RNA |
| Isolation Technology: | Spin Column (Glass Fiber Filter) |
| Product Line: | Ambion™, RNAqueous® |
| Product Size: | 50 preps |
| Shipping Condition: | Room Temperature |
Contents & storage
• 6 ml Formaldehyde Load Dye (-20°C)
• 60 ml Lysis/Binding Solution (4°C)
• 60 ml Water for 64% Ethanol (4°C)
• 4 ml Lithium Chloride Precipitation Solution (4°C)
• 40 ml Wash Solution 1 (4°C)
• 80 ml Wash Solution 2/3 Concentrate (4°C)
• 50 Filter Cartridges (room temperature)
• 100 Collection Tubes (room temperature)
• 10 ml Elution Solution (-20°C, 4°C, or room temperature)
• 60 ml Lysis/Binding Solution (4°C)
• 60 ml Water for 64% Ethanol (4°C)
• 4 ml Lithium Chloride Precipitation Solution (4°C)
• 40 ml Wash Solution 1 (4°C)
• 80 ml Wash Solution 2/3 Concentrate (4°C)
• 50 Filter Cartridges (room temperature)
• 100 Collection Tubes (room temperature)
• 10 ml Elution Solution (-20°C, 4°C, or room temperature)
