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Catalog Number | Color | Excitation Wavelength Range | Dye Type |
---|---|---|---|
B7474 | None | None | Biotin-XX |
A22281 | Blue | 346/442 nm | Alexa Fluor™ 350 |
A30104 | Violet | 405/450 nm | Alexa Fluor™ Plus 405 |
A12379 | Green | 495/518 nm | Alexa Fluor™ 488 |
O7466 | Green | 496/520 nm | Oregon Green™ 488 |
F432 | Green | 496/516 nm | FITC (Fluorescein) |
A22282 | Yellow | 531/554 nm | Alexa Fluor™ 532 |
R415 | Red-orange | 540/565 nm | TRITC (Tetramethylrhodamine Isothiocyanate) |
A22283 | Orange | 556/570 nm | Alexa Fluor™ 546 |
A34055 | Orange | 555/565 nm | Alexa Fluor™ 555 |
A30106 | Orange | 555/565 nm | Alexa Fluor Plus 555 |
B3475 | Red | 558/569 nm | BODIPY™ |
A12380 | Orange-red | 578/600 nm | Alexa Fluor™ 568 |
A12381 | Red | 581/609 nm | Alexa Fluor™ 594 |
T7471 | Red | 591/608 nm | Texas Red™ |
A22284 | Far-red | 632/647 nm | Alexa Fluor™ 633 |
A34054 | Far-red | 633/647 nm | Alexa Fluor™ 635 |
A22287 | Far-red | 650/668 nm | Alexa Fluor™ 647 |
A30107 | Far-red | 650/668 nm | Alexa Fluor Plus 647 |
A22285 | Near-infrared | 663/690 nm | Alexa Fluor™ 660 |
A22286 | Near-infrared | 679/702 nm | Alexa Fluor™ 680 |
A30105 | Near-infrared | 758/784 nm | Alexa Fluor™ Plus 750 |
P3457 | None | None | Phalloidin (unlabeled) |
A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.
Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.
Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining
Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.
Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.
Features of phalloidin probes