Invitrogen™

Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay

Green features
Catalog number: C10289
Invitrogen™

Click-iT™ AHA Alexa Fluor™ 488 Protein Synthesis HCS Assay

Green features
Catalog number: C10289
Catalog Number
C10289
Unit Size
2 plates
Price (USD)
648.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C102892 plates
648.00
Your Price:
***
Product Overview
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The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or “click" reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne. The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min⁄Max format.

Specifications

Color
Blue, Green
Detection Method
Fluorescent
Dye Type
Alexa Fluor™ 488, Hoechst 33258
Emission
UV, Visible
Format
96-well plate
Green Features
Less hazardous
Product Line
Alexa Fluor™, Click-iT™, Molecular Probes™
Shipping Condition
Room Temperature
For Use With (Equipment)
Fluorescence Microscope

Contents & Storage

The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
  • Store at ≤-20°C, desiccate and protect from light.
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