The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay provides a fast, sensitive, non-toxic and non-radioactive method for the detection of nascent protein synthesis utilizing fluorescence microscopy and high-content imaging. Click-iT® AHA is an amino acid analog of methionine containing an azido moiety. Similar to 35S-methionine, Click-iT® AHA is fed to cultured cells and incorporated into proteins during active protein synthesis. Detection of the incorporated amine acid utilizes a chemoselectiove ligation or “click" reaction between an azide and an alkyne, where the azido modified protein is detected with the green-fluorescent Alexa Fluor® 488 alkyne. The Click-iT® AHA Alexa Fluor® 488 Protein Synthesis HCS Assay has been successfully tested in A549 and U-2 OS cells with a variety of reagents that inhibit protein synthesis including cycloheximide, anisomycin and puromycin in both dose response and Min⁄Max format.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Color
Blue, Green
Detection Method
Fluorescent
For Use With (Application)
High-content Screening (HCS)
For Use With (Equipment)
Fluorescence Microscope, High Content Analysis Instrument
Product Type
HCS Assay
Dye Type
Alexa Fluor™ 488, Hoechst 33258
Emission
UV, Visible
Format
96-well plate
Green Features
Less hazardous
Product Line
Alexa Fluor™, Click-iT™, Molecular Probes™
Shipping Condition
Room Temperature
Contents & Storage
The kit contains sufficient reagents for performing 2 plates in a 96-well plate format.
Store at ≤-20°C, desiccate and protect from light.