MegaX DH10B™ T1R Electrocomp™ Cells

Catalog number: C640003

Invitrogen™  Related applications: Transformation

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Description

MegaX DH10B™ T1R Electrocomp™ Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/µg of pUC DNA), making the MegaX DH10B™ T1R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B™ T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards your valuable clones and libraries.

Using MegaX DH10B™ T1R cells
MegaX DH10B™ T1R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems (mcrA Δ(mrr-hsdRMS-mcrBC)). The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.

Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG tonA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Electrocompetent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 3x10^10
Bacterial or Yeast Strain: DH10B™
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Lacks F' Episome
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Plasmid Type: May be used for plasmids > 100 kb
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 25 transformations
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): Yes
Shipping Condition: Dry Ice

Contents & storage

Contains:
• MegaX DH10B™ T1R Electrocomp™ C: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• Recovery Medium: 3 bottles, 10 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store Recovery Medium at 4°C .

Documents

Manuals & protocols

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