Invitrogen™

MegaX DH10B T1R Electrocomp™ Cells

Catalog number: C640003
Invitrogen™

MegaX DH10B T1R Electrocomp™ Cells

Catalog number: C640003
Catalog Number
C640003
Unit Size
100 µL
Price (USD)
391.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C640003100 µL
391.00
Your Price:
***
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MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes now guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/µg of pUC DNA), making the MegaX DH10B T1R cells ideal for highly demanding cloning and library construction. They have the same genotype as the widely used DH10B T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards your valuable clones and libraries.

Using MegaX DH10B T1R cells
MegaX DH10B T1R cells provide highly efficient transformation of methylated DNA, since they lack the E. coli K12 restriction systems (mcrA Δ(mrr-hsdRMS-mcrBC)). The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal.

Genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC), Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG tonA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Tube
For Use With (Application)
Transformation, Cloning
Product Line
DH10B, Electrocomp™
Product Type
Electrocomp Cell
Competent Cell Type
Electrocompetent
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Blue/White Screening
Yes
F'episome (to make ssDNA)
Lacks F' Episome
Cloning Methylated DNA
Yes
Bacterial or Yeast Strain
DH10B
Improves Plasmid Quality
Yes
Propagating ccdB Vectors
Not for ccdB vector propagation
Plasmid
May be used for plasmids > 100 kb
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Shipping Condition
Dry Ice
Reduces Recombination
Yes
Species
E. coli
T1 Phage - Resistant (tonA)
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Transformation Efficiency
> 3x10^10
High-throughput Compatibility
Not High-throughput Compatible (Manual)

Contents & Storage

Contains:
• MegaX DH10B T1R Electrocomp C: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• Recovery Medium: 3 bottles, 10 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store Recovery Medium at 4°C .

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