Cascade Blue™ Hydrazid, Trinatriumsalz

Zitierungen und Referenzen (24)

Invitrogen™

Cascade Blue™ Hydrazid, Trinatriumsalz

Der zellpermeable, fixierbare und polare Tracer Cascade Blue™ Hydrazid kann zur Untersuchung der Zell-Zell- und Zell-Liposomen-Fusion sowie der Membrandurchlässigkeit undWeitere Informationen

Katalognummer	Menge
C687	10 mg

Katalognummer C687

Preis (EUR)

486,00

Zum Warenkorb hinzufügen

Menge:

10 mg

Preis (EUR)

486,00

Zum Warenkorb hinzufügen

Der zellpermeable, fixierbare und polare Tracer Cascade Blue™ Hydrazid kann zur Untersuchung der Zell-Zell- und Zell-Liposomen-Fusion sowie der Membrandurchlässigkeit und zum Transport durch Lückenverbindungen oder die Zellaufnahme während der Pinocytose verwendet werden. Dieser wasserlösliche Tracer kann mittels der Whole-Cell-Patch-Clamp-Technik, Iontophorese, osmotischer Lyse von pinozytischen Vesikeln oder vergleichbare Methoden in die Zellen eingebracht werden. Darüber hinaus können Carbonsäuren von Proteinen und anderen wasserlöslichen Biopolymeren in wässriger Lösung mit wasserlöslichen Carbodiimiden wie EDAC (E2247) an dieses Molekül gekoppelt werden.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Chemische ReaktivitätCarbonsäure, Keton, Aldehyd

Emission412 nm

Anregung399 nm

Marker oder FarbstoffCascade Blue™

Molekulargewicht596.44 G/MOL

ProdukttypHydrazid

Menge10 mg

Reaktiver TeilAmin, Hydrazid

VersandbedingungRaumtemperatur

MarkertypKlassische Farbstoffe

ProduktlinieCASCADE BLUE

Unit SizeEach

Inhalt und Lagerung

Lagerung bei Raumtemperatur und vor Licht geschützt

Häufig gestellte Fragen (FAQ)

What is the recommended concentration for preparation of Cascade Blue hydrazide, Trisodium Salt (Cat. No. C687) stock solution?

We recommend preparing a 1% w/v stock solution in sterile water. Please note that the dye can aggregate in solution over time.

Zitierungen und Referenzen (24)

Zitierungen und Referenzen

Abstract

Parathyroid hormone controls the size of the intracellular Ca(2+) stores available to receptors linked to inositol trisphosphate formation.

Authors:Short AD, Taylor CW

Journal:J Biol Chem

PubMed ID:10636879

'In HEK 293 cells stably expressing type 1 parathyroid (PTH) receptors, PTH stimulated release of intracellular Ca(2+) stores in only 27% of cells, whereas 96% of cells responded to carbachol. However, in almost all cells PTH potentiated the response to carbachol by about 3-fold. Responses to carbachol did not desensitize, ... More

Neuropeptides exert direct effects on rat thymic epithelial cells in culture.

Authors:Head GM, Mentlein R, von Patay B, Downing JE, Kendall MD

Journal:Dev Immunol

PubMed ID:9716910

'To determine if major thymic neuropeptides and neurotransmitters can directly influence the functional activity of cultured rat thymic epithelium, neuropeptides and neurotransmitters were applied, and intercellular communication, proliferation, and thymulin secretion assessed. After injections of a mixture of lucifer yellow dextran (too large to pass gap junctions) and cascade blue ... More

Oligosaccharide organization of the beta-subunits of pig kidney Na+/K+-ATPase.

Authors:Amler E, Staffolani R, Baranska J, Obsil T, Urbanová P, Bertoli E, Mazzanti L

Journal:Physiol Res

PubMed ID:9730046

'The distance between the beta-subunits of Na+/K+-ATPase isolated from pig dark red kidney medulla was determined by Förster energy transfer. First, oligosaccharides of the beta-subunit were shown to be labelled with three fluorophores: Lucifer yellow (LY), Lissamine rhodamine B sulfonyl hydrazine (LRSH) and Cascade blue (CB). Further, LY and LRSH ... More

Site-directed conjugation of nonpeptide groups to peptides and proteins via periodate oxidation of a 2-amino alcohol. Application to modification at N-terminal serine.

Authors:Geoghegan KF, Stroh JG

Journal:Bioconjug Chem

PubMed ID:1325188

'The 2-amino alcohol structure -CH(NH2)CH(OH)- exists in proteins and peptides in N-terminal Ser or Thr and in hydroxylysine. Its very rapid oxidation by periodate at pH 7 generates an aldehyde in the peptide and is the first step in a method for site-directed labeling with biotin or a fluorescent reporter. ... More

Functional analysis of amino acid sequences in connexin43 involved in intercellular communication through gap junctions.

Authors:Becker DL, Evans WH, Green CR, Warner A

Journal:J Cell Sci

PubMed ID:7615666

'Gap junctions allow direct communication between cells without recourse to the extracellular space and have been widely implicated as important mediators of cell-cell signalling. They are constructed from the connexin proteins, which form a large family, and individual connexins show complex spatial and temporal variations in their expression patterns. Understanding ... More

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