Invitrogen™

MultiShot™ StripWell Stbl3™ Competent Cells

Catalog number:  C739601

Related applications:

Transformation

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Description

MultiShot StripWell Stbl3 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a rack containing 12 strips of 8 wells to increase productivity for medium-throughput bacterial transformations. MultiShot StripWell Stbl3 competent E. coli cells are designed especially for cloning direct repeats found in lentiviral expression vectors. Unlike TOP10 E. coli, these cells reduce the frequency of homologous recombination of long terminal repeats (LTRs) found in ViraPower Lentiviral Expression vectors and other retroviral vectors. The DNA yield from Stbl3 cells is often >10-fold higher than alternative strains such as TOP10 (see table).

Reduce unwanted recombination with Stbl3 cells
MultiShot StripWell Stbl3 Competent Cells are derived from the HB101 E. coli strain and are recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats (e.g., ViraPower Lentiviral Expression kits). The transformation efficiency of MultiShot StripWell Stbl3 Competent Cells is greater than 1 × 107 cfu/µg plasmid DNA.

Key features of the Stbl3 Competent Cells include:
Optimal performance for the propagation of lentiviral vectors
mcrB, mrr for enhanced genomic cloning capacity
recA13 for reduced occurrence of non-specific recombination in cloned DNA
High DNA yields

MultiShot StripWell format—flexible for no waste
Each StripWell of eight connected tubes is designed with the same single-tube, single-use features as our One Shot format tubes. This format allows for all steps of the transformation protocol, up to plating, to take place in the same tube, thereby saving time and to preventing contamination. After addition of DNA, MultiShot StripWell Competent Cells can be transformed by heat-shock at 42°C using a water bath, all in the same tube.

The StripWell tubes can be cut to permit transformations in a single tube, eliminating wasted aliquots of cells and avoiding freeze-thaws that can result in reduced transformation efficiency.

Key features of the MultiShot StripWell format include:
Increase productivity—for medium throughput experimentation
Maximal flexibility—transform as few as 1 tube and as many as 96 from the StripWell rack
Familiar convenience—no aliquotting, add DNA directly to cells, heat shock, and recover in the same tube
Peace of mind—single-use transformation minimizes contamination

Genotype: F-mcrB mrr hsdS20(rB-, mB-) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ- leu mtl-1

Find the strain and format that you need
We offer many other E. coli strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot formatted comp cells or email us for custom formatting options.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: Stbl3™
Blue/White Screening: No
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: High-throughput Compatible
Plasmid: May be used for plasmids > 20 kb
Product Line: MultiShot™, Stbl3™
Propagating ccdB Vectors: Not for ccdB vector propagation
Shipping Condition: Dry Ice
Format: 8-well strips
Species: E. coli

Contents & storage

• MultiShot StripWell Stbl3 Competent Cells: One 96-stripwell rack, 50 µL per well, store at –80°C
• pUC19 DNA (10 pg/µL): 1 vial, 50 µL, store at –20°C
• S.O.C. medium: 2 bottles, 15 mL each, store at 4°C or room temperature