Thermo Scientific™

Enterobacteria Enrichment Broth (Dehydrated)

Related applications:

Industrial Microbiology

Selectively enrich bile tolerant Gram-negative bacteria during microbiological examination of pharmaceutical products with Thermo Scientific™ Oxoid™ Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP).
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Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP) is recommended as an enrichment medium for bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products. It can be used as test for absence or as a quantitative test1.
  • Adheres to EP/USP/JP/BP specifications.
Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP) was formulated to overcome the unsatisfactory effects of inhibition on small numbers of bile-tolerant Gram-negative cells due to bile salt variations. The inclusion of purified ox bile eliminated these problems and a preliminary assay can be used to check growth by inoculating approximately one viable cell per medium unit2, 3.

Enterobacteria Enrichment Broth-Mossel (EP/USP/JP/BP) should be used as an enrichment broth in conjunction with Violet Red Bile Glucose Agar (R455302).

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1. European Pharmacopoeia 6.1. 2.6.13 Microbiological Examination of Non-Sterile Products: Test for Specified Micro-organisms. B. Harmonised Method.
2. Mossel D. A. A., Harrewijn G. A. and Nesselrooy-van Zadelhoff C. F. M. (1974) Health Lab. Sci. 11. 260-267.
3. Richard N. (1982) in Quality Assurance and quality control of microbiological culture media. Ed. J.E.L. Corry. G.I.T. - Verlag Darmstadt. pp 51-57.