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CD3D & CD3E Recombinant Human Protein, hIgG1-Fc & hIgG1-Fc.His tag

Catalog number: CT026H0323H5

 Related applications: Protein Assays and Analysis

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Description

CD3D molecule delta and CD3E molecule epsilon (CD3-TCR complex)Heterodimer (CD3D & CD3E), recombinant human protein is supplied as a lyophilized powder.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) fused to the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; a DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) fused to the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.

Activity: This recombinant protein has not been tested.

Formulation: 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: None
Gene ID: 915, 916
Gene Symbol: CD3D & CD3E
Molecular Weight: 77.5 kDa
Product Size: 5 µg
Protein Family: CD Proteins
Protein Form: Recombinant, Truncated
Protein Tag or Fusion: DYKDDDDK, His Tag, IgG1 Fc Tag
Purification: PAGE
Purity: ≥ 90% by SDS-PAGE
Research Category: Immunology, Signal Transduction
Species: Human
Tag Position: C-Terminal
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols