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Citations & References (9)
Invitrogen™
DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)
The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maximaRead more
| Catalog Number | Quantity |
|---|---|
| D6488 also known as D-6488 | 5 mg |
Catalog number D6488
also known as D-6488
Price (JPY)Contact Us ›
96,000
Each
Quantity:
5 mg
The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660). Although the substrate itself is fluorescent (excitation/emission maxima ∼460/610 nm), the difference between the substrate's and the hydrolysis product is over 200 nm, along the two species to be easily distinguished.
To make a concentrated stock solution of up to 5 mg/mL, dissolve in high quality anhydrous DMSO. Store frozen, desiccated and protected from light.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission465/608 nm
Molecular Weight (g/mol)470.31
Quantity5 mg
Shipping ConditionRoom Temperature
SubstrateBeta-Gal Substrate
Detection MethodFluorescence
Substrate PropertiesChemical Substrate
Unit SizeEach
Contents & Storage
Store in freezer (-5°C to -30°C) and protect from light.
Citations & References (9)
Citations & References
Abstract
Oxygen sensitivity of reporter genes: implications for preclinical imaging of tumor hypoxia.
Journal:Mol Imaging
PubMed ID:17711777
'Reporter gene techniques have been applied toward studying the physiologic phenomena associated with tumor hypoxia, a negative prognostic indicator. The purpose of this study was to assess the potential adverse effects of hypoxic conditions on the effectiveness of four commonly used reporter genes: Renilla luciferase, monomeric red fluorescent protein, thymidine
In vivo imaging of beta-galactosidase activity using far red fluorescent switch.
Journal:Cancer Res
PubMed ID:14996712
'beta-Galactosidase (beta-gal) has been widely used as a transgene reporter enzyme, and several substrates are available for its in vitro detection. The ability to image beta-gal expression in living animals would further extend the use of this reporter. Here we show that DDAOG, a conjugate of beta-galactoside and 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) (DDAO),
beta-Galactosidase activity assay using far-red-shifted fluorescent substrate DDAOG.
Journal:Anal Biochem
PubMed ID:19103143
beta-Galactosidase (beta-gal) is commonly used as a reporter gene in biological research, and a wide variety of substrates have been developed to assay its activity. One substrate, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) beta-d-galactopyranoside (DDAOG), can be cleaved by beta-gal to produce 7-hydroxy-9H(I,3-dichloro-9,9-dimethylacridin-2-one) (DDAO). On excitation, DDAO generates a far-red-shifted fluorescent signal. Using this substrate,
Enzymatic detection of protein translocation.
Journal:Nat Methods
PubMed ID:15973423
Fundamental to eukaryotic cell signaling is the regulation of protein function by directed localization. Detection of these events has been largely qualitative owing to the limitations of existing technologies. Here we describe a method for quantitatively assessing protein translocation using proximity-induced enzyme complementation. The complementation assay for protein translocation (CAPT)
Quantification of beta-galactosidase activity after non-viral transfection in vivo.
Journal:J Control Release
PubMed ID:12932652
The limited efficacy of non-viral gene delivery systems currently hampers their wider therapeutic use. In order to further develop novel gene delivery systems, it is important to quantify their efficacy. Many reporter gene assays have limitations when being used to quantify expression in vivo. We have developed a simple assay