Remel™ PathoDxtra™ Strep Grouping Kit

Thermo Scientific™  Related applications: Clinical Microbiology

Eliminate the need for incubation and provide a faster strep grouping solution than more traditional enzyme extraction with the Thermo Scientific™ PathoDxtra™ Strep Grouping Kit.

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Description

  • Improves workflow efficiencies—instant acid extraction without the need for incubation or reconstitution
  • Saves time—simple, rapid procedure produces easy-to-read results
  • Reduces costs—identifies all clinically important Strep groups, including Group D; no additional reagents required
  • Provides flexibility—pack variants available to suit testing needs

Includes:

PathoDxtra Strep Grouping Kit (Mfr. No. DR0700M): 3mL each of A, B, C, D, F and G grouping latex; Polyvalent Positive Control, 2.8mL; Extraction Reagent 1: 4mL; Extraction Reagent 2: 4mL; Extraction Reagent 3: 2 x 10mL; Mixing Sticks; Disposable Reaction Cards
 

PathoDxtra Extraction Reagent Set (Mfr. No. DR0709M): Extraction Reagent 1: 4mL; Extraction Reagent 2: 4mL; Extraction Reagent 3: 2 x 10mL; For use with the PathoDxtra Strep Grouping Kit

PathoDxtra Strep Grouping Reagent Set (Mfr. No. DR0710M): Extraction Reagent 1 - 4mL; Extraction Reagent 2 - 4mL; Extraction Reagent 3 - 2 x 10mL; Mixing Sticks, Disposable Reaction Cards; For use with selected, separately purchased PathoDxtra Strep Grouping latex reagents

Rapid Streptococcal grouping tests correlate best with reference methods when only Streptococci that are Β-hemolytic on sheep blood agar are tested. 1, 2, 3, 4


The latex particles of the PathoDxtra grouping reagents are coated in relevant specific antibodies. These react with any corresponding Streptococcal group antigen extracted from the bacterial cell wall, causing agglutination. The test works with as little as one, but up to four, colonies of an 18 to 24 hour culture of β-hemolytic Streptococcal isolates. Rapid reactions are due to group-specific antigen being extracted instantaneously using the room-temperature, nitrous acid extraction procedure. Once neutralized, the extracted antigen is tested against specific IgG-coated latex particles on a test slide. The distinct and easily recognizable granular agglutination pattern of a positive test contrasts markedly with the uniform, milky appearance of a negative result, making the test simple to interpret. The latex particles of the PathoDxtra grouping reagents are coated in the relevant specific antibodies. These react with any corresponding streptococcal group antigen extracted from the bacterial cell wall, causing agglutination. The test works with as little as one, but up to four, colonies of an 18 to 24 hour culture of β-hemolytic streptococcal isolates. Rapid reactions are due to group-specific antigen being extracted instantaneously using the room-temperature, nitrous acid extraction procedure. Once neutralized, the extracted antigen is tested against specific IgG-coated latex particles on a test slide. The distinct and easily recognizable granular agglutination pattern of a positive test contrasts markedly with the uniform, milky appearance of a negative result, making the test simple to interpret.

General References:

1. Murray, P.R., Baron, E.J., Jorgensen, J.H. et al. (2003) Manual of Clinical Microbiology. 8th ed., Vol 1. ASM, Washington, D.C.

2. Evins, G.M., et al. (1983) The development by the Centers for Disease Control of a specification for streptococcal serogrouping kits and its application to Streptex and to the Phadebact Streptococcus Test. J. Biol. Standard. 11:333-339.

3. Facklam, R.R., et al. (1979) Evaluation of commercial latex agglutination reagents for grouping streptococci. J. Clin. Microbiol. 10:641-646.

4. Slifkin, M., and Pouchet-Melvin, G.R. (1980) Evaluation of three commercially available test products for serogrouping betahemolytic streptococci. J. Clin. Microbiol. 11:249-255.

Rapid Streptococcal grouping tests correlate best with reference methods when only Streptococci that are Β-hemolytic on sheep blood agar are tested. 1, 2, 3, 4


The latex particles of the PathoDxtra grouping reagents are coated in relevant specific antibodies. These react with any corresponding Streptococcal group antigen extracted from the bacterial cell wall, causing agglutination. The test works with as little as one, but up to four, colonies of an 18 to 24 hour culture of β-hemolytic Streptococcal isolates. Rapid reactions are due to group-specific antigen being extracted instantaneously using the room-temperature, nitrous acid extraction procedure. Once neutralized, the extracted antigen is tested against specific IgG-coated latex particles on a test slide. The distinct and easily recognizable granular agglutination pattern of a positive test contrasts markedly with the uniform, milky appearance of a negative result, making the test simple to interpret. The latex particles of the PathoDxtra grouping reagents are coated in the relevant specific antibodies. These react with any corresponding streptococcal group antigen extracted from the bacterial cell wall, causing agglutination. The test works with as little as one, but up to four, colonies of an 18 to 24 hour culture of β-hemolytic streptococcal isolates. Rapid reactions are due to group-specific antigen being extracted instantaneously using the room-temperature, nitrous acid extraction procedure. Once neutralized, the extracted antigen is tested against specific IgG-coated latex particles on a test slide. The distinct and easily recognizable granular agglutination pattern of a positive test contrasts markedly with the uniform, milky appearance of a negative result, making the test simple to interpret.

General References:

1. Murray, P.R., Baron, E.J., Jorgensen, J.H. et al. (2003) Manual of Clinical Microbiology. 8th ed., Vol 1. ASM, Washington, D.C.

2. Evins, G.M., et al. (1983) The development by the Centers for Disease Control of a specification for streptococcal serogrouping kits and its application to Streptex and to the Phadebact Streptococcus Test. J. Biol. Standard. 11:333-339.

3. Facklam, R.R., et al. (1979) Evaluation of commercial latex agglutination reagents for grouping streptococci. J. Clin. Microbiol. 10:641-646.

4. Slifkin, M., and Pouchet-Melvin, G.R. (1980) Evaluation of three commercially available test products for serogrouping betahemolytic streptococci. J. Clin. Microbiol. 11:249-255.



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