E-Gel® SizeSelect™ Agarose Gels, 2%

Catalog number: G661002

Invitrogen™  Related applications: SOLiD® Next-Generation Sequencing

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Description

The E-Gel® SizeSelect™ Gels are the most convenient method for the purification of DNA libraries for Next Generation Sequencing applications. With E-Gel® SizeSelect™ gels, you can separate and recover DNA for library construction in 3 easy steps: Load, Run, and Retrieve. Advantages of the E-Gel® SizeSelect™ Gels:

• Save time—avoid additional gel purification steps required by other methods
• Simplify DNA recovery—remove purified DNA directly from the well with a pipette
• Improve sequencing results—minimize DNA damage by using blue-light transillumination
• Improve result consistency—eliminate tedious and error-prone gel extraction steps

How it works
The E-Gel® SizeSelect gel features two rows of wells. To extract a DNA band of interest without conventional gel purification, simply load your sample into the top row and electrophorese until your band moves into the bottom row of wells. Then, simply use a pipette to remove your purified DNA in the appropriate size range. Guides on the gel cassette help you track your target DNA band so you will know when it is ready for retrieval.

Better results
The direct access to your sample from the retrieval well (see figure) allows you to avoid the need to excise gel band slices and risk losing your DNA during a gel purification step.

A self-contained power system
E-Gel® SizeSelect™ gels run in the E-Gel® iBase™ Power System (see E-Gel Electrophoresis System image). This self-contained device includes a built-in power supply. Simply connect the AC adaptor provided and plug into an electrical outlet. View program selection and running time on the easy-to-read LCD display. Preset programs are available for various gel types or you can manually set your own run times. A reverse program allows you to change the field direction, ensuring complete capture of DNA. In addition, the EGel® iBase™ Power System includes an automatic shutoff feature to ensure that you will not overrun your gel. The E-Gel® iBase™ Power System fits on a Safe Imager™ Blue-Light Transilluminator, which allows you to safely monitor DNA bands migrate in real-time. Blue-light transillumination, such as ones found in the E-Gel® Imager and the Safe Imager™ 2.0 Blue-Light Transilluminator, can be used to view the proprietary stain used in the E-Gel® SizeSelect™ gels. This gives the greater safety to the user and prevents DNA damage associated with the use of ethidium bromide and UV transillumination.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Wells: 2 x 8 wells
System: E-Gel CloneWell
Gel Type: E-Gel®
Quantity: 10 gels
In-Gel Dye: Proprietary Stain
Well Format: CloneWell
Product Size: 10⁄pack
Gel Percentage: 2 %
Separation Range: 50 bp to 1 kb
Mode of Separation: Size
Sample Loading Volume: 25 µl
Sample Type (Specific): Double-Stranded DNA (dsDNA)
Shipping Condition: Room Temperature

Contents & storage

10 E-Gel® SizeSelect™ 2% Agarose - store at room temperature

Documents

Manuals & protocols

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