Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg/mL Solution in Water
Description
Hoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant.Figures
HeLa cell nuclei. Texas Red-X streptavidin, biotin-XX goat anti–mouse IgG antibody, ELF 97 Cytological Labeling Kit and Hoechst 33258.
Exponentially growing promyelocytic leukemia (HL60) cells. Hoechst 33258 and ChromaTide BODIPY FL-14-dUTP.
A zebrafish retina cryosection labeled with the mouse monoclonal antibody FRet 43 and detected using TSA Kit #2 with the HRP conjugate of goat anti–mouse IgG antibody and Alexa Fluor® 488 tyramide.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell (BPAEC).
Madin-Darby canine kidney (MDCK) cells.
Normal and camptothecin-treated bovine pulmondary artery endothelial cells.
Mouse anti-a-Tubulin-Alexa 488
A J774 mouse macrophage cell stained with BODIPY® FL ganglioside GM1 (B13950) and Alexa Fluor® 555 dye–labeled cholera toxin subunit B.
Detection of BrdU-incorporating cells using the SBIP technique.
Immunofluorescence using Claudin-4 Monoclonal Antibody, Mouse, Alexa Fluor® 488 Conjugate
In situ hybridization of a-satellite probes to human chromosomes 1, 15 and 17 detected by tyramide signal amplification.
Ovarioles from Drosophila melanogaster.
Live bovine pulmonary artery endothelial cells (BPAEC) stained with LysoTracker® Red DND-99, dihydrorhodamine 123 and Hoechst 33258.
Nucleus and Golgi apparatus of a bovine pulmonary artery endothelial cell labeled with Hoechst 33342 and the BSA complex of BODIPY® FL C5-ceramide.
Specifications
| Excitation/Emission: | 352⁄461 |
|---|---|
| Form: | Liquid |
| Label or Dye: | Hoechst 33258 |
| Shipping Condition: | Room Temperature |
| Sub Cellular Localization: | Nucleus, Nucleic Acids |
| Detection Method: | Fluorescent |
| For Use With (Equipment): | Fluorescence Microscope |
| Color: | Blue |
| Cell Permeability: | Cell-Permeant |
