Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg/mL Solution in Water

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Hoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant.
For Research Use Only. Not for use in diagnostic procedures.


Excitation/Emission: 352⁄461
Form: Liquid
Label or Dye: Hoechst 33258
Shipping Condition: Room Temperature
Sub Cellular Localization: Nucleus, Nucleic Acids
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope
Color: Blue
Cell Permeability: Cell-Permeant

Contents & storage

Store in refrigerator (2–8°C) and protect from light.