Células HepaRG™, crioconservadas
Células HepaRG™, crioconservadas
Gibco™

Células HepaRG™, crioconservadas

La línea celular HepaRG™ es una línea celular hepática inmortalizada que conserva muchas características de los hepatocitos humanos primarios. LasMás información
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Número de catálogo HPRGC10
Precio (MXN)
25,542.80
Each
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Precio (MXN)
25,542.80
Each
Añadir al carro de la compra
La línea celular HepaRG™ es una línea celular hepática inmortalizada que conserva muchas características de los hepatocitos humanos primarios. Las células HepaRG™ se diferencian terminalmente y se proporcionan en un cómo formato criopreservado. Para científicos que necesitan datos reproducibles del metabolismo, las células HepaRG™ son una herramienta in vitro que proporciona resultados reproducibles en un sistema metabólicamente completo y ampliable.
• Consiga resultados biológicamente relevantes de un sistema metabólicamente completo
• Evalúe el potencial de interacción fármaco - fármaco de su compuesto
• Consiga resultados reproducibles de una sola población de células

Biológicamente relevantes
Las células HepaRG™ presentan muchas características de los hepatocitos humanos primarios, incluyendo morfología, expresión de enzimas metabólicas clave, expresión de receptores nucleares y transportadores de fármacos. A diferencia de las células HepG2 y Fa2N-4, las células HepaRG™ tienen una alta actividad P450 y una expresión completa de todos los receptores nucleares.

Prevea la interacción fármaco - fármaco basada en el metabolismo
Las células HepaRG™ responden a los inductores e inhibidores prototípicos P450 en la misma medida que los hepatocitos primarios, permitiendo que las células HepaRG™ se utilicen para evaluar las posibles interacciones fármaco - fármaco.

Reproducibles infinitamente
Las células HepaRG™ presentan muchas características de los hepatocitos humanos primarios y son esencialmente de un solo donante. Estas características permiten a los usuarios obtener resultados fisiológicos relevantes para las interacciones fármaco - fármaco basadas en el metabolismo sin la preocupación de la variabilidad de los donantes y los tamaños de lote limitados que dependen del tejido del donante. (Nota: Las células que proporcionamos están terminalmente diferenciadas.

HepaRG™ es una marca comercial de BioPredic International.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Fuente de dadoresDonante único
N.° de celdas10 x 10^6
Tipo de productoHepatocitos
Cantidad1 vial(es)
Condiciones de envíoHielo seco
AgeAdulta
Línea de célulasHepaRG™
Tipo de célulaCélula de hepatocitos
FormularioCrioconservado
SexoMujer
EspecieHumano
Unit SizeEach

Preguntas frecuentes

I have a sub-optimal monolayer confluency for my HepaRG cells. What should I do?

Here are possible causes and suggestions:

-Low seeding density: Follow recommended procedure for seeding the cells depending upon your application in the HepaRG Cell User Guide (http://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html).
-Low attachment efficiency; inadequate time allowed for attachment
--Low attachment efficiency may be due to:
---Poor-quality matrix: Use only Collagen I coated plates from recognized manufacturers.
---Cells not handled gently: Freezing and thawing procedures are stressful for cells and cause them to be fragile. Handle the cells gently.

I have an unexpected cell number for my HepaRG cells. Why is this?

Here are possible reasons:

-Improper thawing technique: Review Section 2.1: Protocol for thawing of HepaRG cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#3).
-Thawing medium is not correct: Use the recommended medium - Review Section 1: Recommended materials, media, and cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#2).
-Centrifugation speed is not correct: Follow the recommended speed for centrifugation. Review Section 2.1: Protocol for thawing of HepaRG cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#3). Make sure that the centrifuge is calibrated. If the viability is very high but the cell number is low, then the centrifugation speed is too low - increase the speed and repeat centrifugation (this may require some optimization). By contrast, if the viability is lower than expected and the number of cells is very high, then the centrifugation speed is too high and is pelleting dead cells as well as viable cells.
-Loss of cells: After centrifugation, aspirate the supernatant while leaving a small volume on the pellet. Be careful not to aspirate the pellet.
-Improper counting technique: Review Section 2.2: Protocol for counting of HepaRG cells (https://www.thermofisher.com/us/en/home/references/protocols/drug-discovery/adme-tox-protocols/heparg-cell-user-guide.html#3). Ensure a homogeneous cell mixture prior to counting. Due to the normal presence of aggregates in the cell suspension, we recommend counting the cells with a hemocytometer.

My HepaRG cells have low post-thaw cell viability. Why is this?

Here are possible reasons:

-Storage temperature not maintained below -80 degrees C or repeated transient increase in temperature to RT
-Cells were thawed incorrectly
-Thawing medium is not correct or was at the incorrect temperature
-Cells were not handled gently
-Counting difficulty: Perform a repeat count with a new sample; avoid vortexing the cells to mix them
-Shipment temperature not maintained below -70 degrees C

After I use the HepaRG cells for an experiment, can I re-use them?

The cells are licensed for single-use applications.

How should the HepaRG cells be stored?

Upon receipt, cryovials must be stored in liquid or vapor phase nitrogen.

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