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Image-iT™ TMRM Reagent (mitochondrial membrane potential indicator)

Catalog number:  I34361

 Related applications:

Cellular Imaging

| Flow Cytometry

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Healthy mitochondrial membranes maintain a difference in electrical potential between the interior and exterior of the organelle, referred to as a membrane potential. Tetramethylrhodamine, methyl ester (TMRM) is a cell-permeant dye that accumulates in active mitochondria with intact membrane potentials. If the cells are healthy and have functioning mitochondria, the signal will be bright. Upon loss of the mitochondrial membrane potential, TMRM accumulation will cease and the signal will dim or disappear. TMRM signal can be detected with fluorescence microscopy, flow cytometry, cell sorting, high-throughput screening, and high content analysis.

• For live-cell dynamic study of mitochondrial membrane potential (not fixable)
• Detectable with RFP/TRITC filter set (absorbance peak: 548 nm, emission peak: 574 nm)
• Mutiplexable with green, deep red, violet, and blue emission dyes, such as GFP, DAPI, Hoechst, HCS LipidTOX, and LysoTracker Deep Red

For Research Use Only. Not for use in diagnostic procedures.


Sub-Cellular Localization: Mitochondria
Color: Orange
Product Line: Molecular Probes™
Product Size: 5 x 100 µL
Shipping Condition: Wet Ice

Contents & storage

Store in freezer (-5 to -30°C) and protect from light.


Manuals & protocols