GeneBLAzer® EDG3-Gα15 HEK 293T DA Assay Kit

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Description

This cell line is available as dividing cells by requesting a quote for K1234 from dividingcells@invitrogen.com

GeneBLAzer® EDG3-Gα15 HEK 293T DA (Division Arrested) cells and EDG3-Gα15-NFAT-bla HEK 293T cells contain the human EDG3 receptor stably integrated in the GeneBLAzer® Gα15-NFAT-bla HEK 293T cell line. GeneBLAzer® Gα15-NFAT-bla HEK 293T cells (#K1212) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element, and stably express the promiscuous G-protein, Gα15. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both EDG3-Gα15 HEK 293T DA cells and EDG3-Gα15-NFAT-bla HEK 293T cells are functionally validated for Z’-factor and EC50 concentrations of Sphingosine-1-phosphate (S1P), (Figure 1). In addition, EDG3-Gα15-NFAT-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

EDG-3(Endothelial-differentiation-gene-3)/S1P-3(Sphingosine-1-Phosphate-3) is a Gq/Gi/Go/G13/G12 coupled GPCR. EDG-3 has been shown to be responsible for bradycardia that has been induced in clinical trials as a side effect of the immunosuppressive drug FTY720 that targets EDG-1. EDG-3 has also been shown to induce vasodilation in response to sphingosylphosphorylcholine (SPC), sphingosine-1-phosphate (S1P), and lysosulfatide (LSF). The EDG-3 Receptor has also been shown to play a cooperative or redundant role in angiogenesis with EDG-1

Specifications

Serum Treatment: Standard (Sterile-Filtered)
Purity or Quality Grade: Qualified
Country of Origin: United States
Format: Bottle(s)
Age: Fetal
Species: Cattle⁄Bovine
Product Size: 1,000 mL
Endotoxin Level: ≤ 10 EU/ml
Hemoglobin Level: ≤ 25 mg⁄dl
Green Features: Fewer resources used, Less waste
Shipping Condition: Dry Ice

Contents & storage




Storage:

GeneBLAzer® EDG3-Gα15 HEK 293T DA Cells and EDG3-Gα15-NFAT-bla HEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer® EDG3-Gα15 HEK 293T DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
Includes:
•EDG3-Gα15 HEK 293T DA cells (K1319A)
•LiveBLAzer™-FRET B/G Loading Kit, 70µg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer® EDG3-Gα15 HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•EDG3-Gα15 HEK 293T DA cells (K1320A)
•1 protocol
•Certificate of Analysis
GeneBLAzer® EDG3-Gα15-NFAT-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•EDG3-Gα15-NFAT-bla HEK 293T cells (K1234)
•1 protocol
•Certificate of Analysis

Documents

Manuals & protocols