Thermo Scientific™

GeneBLAzer™ ER Beta DA Assay Kit

Catalog number:  K1399

Related applications:

Cellular Imaging

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Description

GeneBLAzer™ER beta DA(Division Arrested) cells and ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER beta DA cells and ER beta-UAS-bla GripTite™ 293 cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™ 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Alternate Agonists: Cholic Acid, Deoxycholic Acid
Antagonists: (z)-guggulsterone
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: 293 T (HEK)
Cell State: Division-Arrested Cells
Detection Method: Fluorescent
Druggable Target: Nuclear Receptors
Gene ID (Entrez): 2100
Gene Symbol: ERβ
Primary Agonist: Chenodeoxycholic Acid (CDCA)
Product Line: GeneBLAzer™
Reporter Gene: BLA (Beta-Lactamase)
System Type: GeneBLAzer™
Target Entry: ER beta, ESR2, ERB, NR3A2

Contents & storage

Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER beta HEK 293 T DA cells (K1399A)LiveBLAzer™-FRET B/G Loading Kit, 70 µg