Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The GeneBLAzer™ C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells contain the human Complement Component 5a Receptor 1 (C5AR1) (Accession No. NM_000710) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 (Cat. No. K1213) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element and a promiscuous G Protein, Gα15, stably integrated into CHO-K1 cells.
The C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells cells are functionally validated for Z'-factor and EC50 concentrations using C5a. In addition, C5AR1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.