GeneBLAzer® C5AR1-Gα15 CHO-K1 DA Assay Kit

Catalog number: K1544

 Related applications: Pharma & Biopharma | Target & Lead Identification & Validation

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The GeneBLAzer® C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells contain the human Complement Component 5a Receptor 1 (C5AR1) (Accession # NM_000710) stably integrated into the GeneBLAzer® Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer® Gα15-NFAT-bla CHO-K1 (Cat # K1213) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element and a promiscuous G Protein, Gα15, stably integrated into CHO-K1 cells.

The C5AR1-Gα15 CHO-K1 DA (Division Arrested) cells cells are functionally validated for Z'-factor and EC50 concentrations using C5a. In addition, C5AR1-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.


System: GeneBLAzer®
Reporter: BLA (Beta-Lactamase)
Cell Line: CHO-K1
Cell State: Division-Arrested Cells
Gene Symbol: C5aR1
Product Size: 1 plate
Accession Number: NM_001736.2
Detection Method: Fluorescent
Druggable Target: GPCRs
Population Status: Single Cell Clone Origin

Contents & storage

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
o GeneBLAzer® C5AR1-G?15 CHO-K1DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o GeneBLAzer® C5AR1-G?15 CHO-K1Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The GeneBLAzer® C5AR1-G?15 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.


Manuals & protocols