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Champion™ pET SUMO Expression System

Catalog number: K30001

Invitrogen™  Related applications: Protein Expression

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Description

The Champion™ pET SUMO Expression System produces the highest levels of soluble protein in E. coli. It utilizes a small ubiquitin-related modifier (SUMO) fusion, belonging to the growing family of ubiquitin-related proteins, to enhance the solubility of expressed fusion proteins. In contrast to ubiquitin, SUMO is involved in the stabilization and localization of proteins in vivo. After expression, the 11 kd SUMO moiety can be cleaved by the highly specific and active SUMO (ULP-1) protease at the carboxyl terminal, producing a native protein*. The Champion™ pET SUMO Protein and Peptide Expression System offers:

• Greatly enhanced solubility with an N-terminal SUMO fusionHighly efficient cleavage- produces native protein of interest with SUMO (ULP-1) protease*Highly specific cleavage- eliminates the chance of your protein of interest being internally digested, regardless of its amino acid sequenceSignificantly increased stability with SUMO fusion-can be used for small peptide productionT7lac promoter for high-level protein expressionN-terminal 6xHis tag for protein detection and purification
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Expression: E. coli
Promoter: T7 and lacO
Bacterial or Yeast Strain: BL21(DE3)
Antibiotic Resistance (Bacterial): Kanamycin (KanR)
Cleavage: SUMO
Cloning Method: TA Cloning®
Constitutive or Inducible System: Inducible
Expression Mechanism: Cell-Based Expression
Inducing Agent: IPTG
Product Size: 20 reactions
Protein Tag or Fusion: His Tag (6x), SUMO Tag
Selection Agent (Eukaryotic): None
Vector Type: pET

Contents & storage

The Champion™ pET SUMO Expression System is provided as a complete system. The Champion™ pET SUMO TA Cloning® box contains linearized Champion™ pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. Store at -20°C. The SUMO Protease box contains SUMO Protease and buffers. Store at -80°C. The One Shot® TOP10 box contains twenty-one 50 µl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C. The One Shot¤ BL21(DE3) box contains twenty-one 50 µl aliquots of chemically competent E. coli, S.O.C. medium, and a control plasmid. Store at -80°C.

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