Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

Filmtracer™ LIVE/DEAD™ Biofilm Viability Kit

Catalog number:  L10316

 Related applications:

Microbiological Analysis

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The FilmTracer™ LIVE⁄DEAD Biofilm Viability kit provides a two-color fluorescence assay of bacterial viability, based on membrane integrity, that has proven usefule for a diverse array of bacterial genera including those growing in biofilm communities. The LIVE⁄DEAD Biofilm Viability kit utilizes mixtures of our SYTO® 9 green fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy bacterial cells. When used alone, the SYTO® 9 stain generally labels all bacteria in a population—those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only bacteria with damaged membranes, causing a rduction in the SYTO® 9 stain fluorescence with both dyes are present. Thus, with an appropriate mixture of the SYTO™ 9 and propdium iodide stains, bacteria with intact cell membranes stain fluorescent green, whereas bacteria with damaged membranes stain fluorescent red.
For Research Use Only. Not for use in diagnostic procedures.


Color: Green, Red
Detection Method: Fluorescent
Emission Class: Visible
Excitation Class: Visible
Excitation⁄Emission (nm): 480⁄500, 635
Flow Cytometer Laser Lines: 488
For Use With (Equipment): Confocal Microscope, Flow Cytometer, Fluorescence Microscope, Fluorescent Imager, Microplate Reader
Form: Liquid
Label or Dye: SYTO® 9, propidium iodide
Product Line: FilmTracer™, Molecular Probes®
Product Size: 1 kit
Sub-Cellular Localization: Cytoplasm & Cytosol
Shipping Condition: Room Temperature

Contents & storage

2 ingredients, store in freezer (-5°C to -30°C); protect from light


Manuals & protocols