LSIVet™ Tetravalent Calf Enteritis Feces ELISA Kit

Catalog number: LSIDTT1

 Related applications: Animal Health

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Description

Same as LSIDTT

Two-well antigen-capture ELISA kits enable sensitive and specific detection of pathogens in animal samples, allowing for reliable and rapid screening and detection of infected animals.

How the kit works:

1. Samples are added to the microplate coated with anti-pathogen antibodies. If present in the sample, specific antigen binds to the anti-pathogen antibodies and form antigen-antibody complexes.
2. After washing, an anti-pathogen peroxidase-labeled conjugate is added, which binds to any antigens attached to the plate.
3. After a second washing, a chromogenic substrate is added. Color development results from oxidation of the substrate by the conjugated peroxidase.
4. After stopping the reaction, results are read using an ELISA plate reader. Positive samples will show color. The amount of color in each well is proportional to the amount of specific antigen in the sample.

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

1. U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009; found at: www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf.

2. World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11; found at: www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf.
For Veterinary Use Only.

Specifications

Target Organism Class: Cryptosporidium spp., E. coli (K99 factor), Rotavirus A, Bovine Coronavirus (BCV)
Validated Application: ELISA
Detection Method: Colorimetric
Sample Type (Specific): Feces
Species: Cattle⁄Bovine, Sheep⁄Ovine, Goat⁄Caprine, All Sensitive Animals
Product Size: 12 tests

Documents

Manuals & protocols