Applied Biosystems™

MethylCode™ Bisulfite Conversion Kit

Catalog number: MECOV50
Have Questions?
Applied Biosystems™

MethylCode™ Bisulfite Conversion Kit

Catalog number: MECOV50
Have Questions?
Catalog Number
MECOV50
Unit Size
50 rxns
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
MECOV5050 rxns
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations
The MethylCode™ Bisulfite Conversion Kit is a fast and easy way to convert unmethylated cytosines from a DNA sample into uracils while methylated cytosines remain unchanged.

The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing, and microarrays. With the MethylCode™ Bisulfite Conversion Kit you can expect:

–Simple, rapid cytosine methylation detection

–Consistency across most experimental conditions
–Streamlined conversion of unmethylated cytosines into uracil, thanks to a coupled DNA denaturation/bisulfite treatment step
–Ultra-pure DNA ideal for subsequent molecular-based analyses



Kit specifications:

–Protocol: Rapid heat denaturation of DNA followed by sodium bisulfite modification with spin column purification

–Sample sources: Plasmid, genomic, and endonuclease digested DNAs
–Input DNA: 500 pg-2 μg DNA per treatment, with 200-500 ng being optimal
–Recovery volume: Purified, bisulfite-treated DNA is recovered in 10 μl elution buffer



Contents and storage:
The MethylCode™ Bisulfite Conversion Kit contains all the reagents necessary to convert your DNA for methylation studies. The kit includes CT Conversion Reagent, Dilution Buffer, Resuspension buffer, Binding Buffer, Wash Buffer, Desulphonation Buffer, Elution Buffer, Spin Columns, Collection Tubes.

Store at room temperature for up to 24 months.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Kit, Bisulfite Conversion
Sample Type
DNA (Genomic)
Shipping Condition
Room Temperature
Starting Material Cell No.
Purified genomic DNA - 200 ng to 500 ng
Format
Kit
Product Line
MethylCode™
Quantity
50 Reactions

Contents & Storage

The MethylCode™ Bisulfite Conversion Kit contains all the reagents necessary to convert your DNA for methylation studies. The kit includes CT conversion reagent, dilution buffer, resuspension buffer, binding buffer, wash buffer, desulphonation buffer, elution buffer, spin columns, and collection tubes.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text