The MethylCode™ Bisulfite Conversion Kit is a fast and easy way to convert unmethylated cytosines from a DNA sample into uracils while methylated cytosines remain unchanged.
The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing, and microarrays. With the MethylCode™ Bisulfite Conversion Kit you can expect:
–Simple, rapid cytosine methylation detection
–Consistency across most experimental conditions
–Streamlined conversion of unmethylated cytosines into uracil, thanks to a coupled DNA denaturation/bisulfite treatment step
–Ultra-pure DNA ideal for subsequent molecular-based analyses
–Protocol: Rapid heat denaturation of DNA followed by sodium bisulfite modification with spin column purification
–Sample sources: Plasmid, genomic, and endonuclease digested DNAs
–Input DNA: 500 pg-2 µg DNA per treatment, with 200-500 ng being optimal
–Recovery volume: Purified, bisulfite-treated DNA is recovered in 10 µl elution buffer
Contents and storage:
The MethylCode™ Bisulfite Conversion Kit contains all the reagents necessary to convert your DNA for methylation studies. The kit includes CT Conversion Reagent, Dilution Buffer, Resuspension buffer, Binding Buffer, Wash Buffer, Desulphonation Buffer, Elution Buffer, Spin Columns, Collection Tubes.
Store at room temperature for up to 24 months.
For Research Use Only. Not for use in diagnostic procedures.