Thermo Scientific™

Lab Vision™ Tyrosinase Ab-1, Mouse Monoclonal Antibody

Related applications:

Anatomical Pathology

Provide accurate, reproducible results with the Thermo Scientific™ Tyrosinase Ab-1, Mouse Monoclonal Antibody.
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Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients.

Host Species: Mouse

Clone: T311

Isotype: IgG2a

Species Reactivity: Human, Dog and Cat. Weakly cross-reacts with Mouse. Others not known.

Epitope: aa 1-433

Immunogen: Recombinant tyrosinase protein

Molecular Weight: 70-80kDa

Positive Control: Melanoma cell lines and Melanoma

Cellular Localization: Cytoplasmic Ab-1 shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Staining of melanomas with Ab-1 showed tyrosinase in melanotic as well as amelanotic variants. Ab-1 is a useful marker for melanocytes and melanomas. Occasionally a minor band at 55kDa is also detected in Western Blotting

Recommended for:

  • Immunohistochemistry (Formalin/paraffin)
  • Western Blotting
  • Immunofluorescence
  • Immunocytology
General References:

1. Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.