Invitrogen™

TaqMan™ Reverse Transcription Reagents

Catalog number: N8080234
Have Questions?
Invitrogen™

TaqMan™ Reverse Transcription Reagents

Catalog number: N8080234
Have Questions?
Catalog Number
N8080234
Unit Size
200 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
2,000 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
N8080234200 reactions
-
43041342,000 reactions
-
Product Overview
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TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.

Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.

Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions

Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Kit
Technique
Reverse Transcription
Optimal Reaction Temperature
42°C
Reverse Transcriptase
MultiScribe
Ribonuclease H Activity
Yes
Shipping Condition
Dry Ice
For Use With (Application)
Real Time PCR (qPCR)
Concentration
50 U/μL
Final Product Type
First-Strand cDNA
No. of Reactions
200 Reactions
Reaction Format
Separate components
Reagent Type
Reverse Transcription
Size (Final Product)
Up to 5 kb
Starting Material
RNA

Contents & Storage

• MultiScribe Reverse Transcriptase, 100 μL (50 U/μL)
• RNase Inhibitor, 100 μL (20 U/μL)
• dNTP Mixture, 1 mL (2.5 mM each dNTP)
• Oligo (dT)16, 100 μL (50 μM)
• Random Hexamers, 100 μL (50 μM)
• 10X RT Buffer, 1.5 mL
• MgCl2 Solution, 1.5 mL

Store at –15 to –25°C.

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