The LanthaScreen™ terbium (Tb)-labeled anti-biotin antibody is a generic reagent that can be used to easily label biotinylated biomolecules for use in TR-FRET assays. Although the terbium chelate donor can be directly attached to a biomolecule such as a protein or peptide, it can also be indirectly attached to a target molecule through a biotin-mediated association, in the form of a Tb-labeled streptavidin or anti-biotin antibody. Invitrogen’s Tb-labeled biotin-binding reagents are universal reagents that easily label biotinylated biomolecules with terbium for use in TR-FRET assays for proteases, kinases, etc.
Contents and Storage:
Each LanthaScreen™ Tb-labeled anti-biotin antibody is supplied in HEPES-buffered saline. The kit also includes 400 µl of a fluorescein-labeled positive control and 100 ml of TR-FRET dilution buffer. Store components as indicated in the product Certificate of Analysis (-20°C, or +20-30°C).
For Research Use Only. Not for use in diagnostic procedures.