Thermo Scientific™

GFP-4E-BP1

Catalog number:  PV4759

Related applications:

Protein Assays and Analysis

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Description

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).

For use in combination with the LanthaScreen® Tb-anti-4E-BP1 [pThr46] Antibody (PV4757) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Entry: Biochemical terbium phospho-specific activity
Detection Method: Fluorescent
Label or Dye: GFP (EmGFP)
Shipping Condition: Dry Ice
Substrate: 4E-BP1
Substrate Properties: Protein-Based Substrate
Target Entry: mTOR, FRAP1
Target Enzyme: Serine⁄Threonine Kinases
Validated Kinases: FRAP1 (mTOR), FRAP1 (mTOR)
Substrate Type: Kinase Substrate
Molecular Weight: 45600 Da

Contents & storage

GFP-4E-BP1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 2mM DTT. Store at -80°C.