4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).
For use in combination with the LanthaScreen® Tb-anti-4E-BP1 [pThr46] Antibody (PV4757) to evaluate inhibitors in medium to high throughput screening applications. An optimized mTor assay buffer (PV4794) is also available.
For Research Use Only. Not for use in diagnostic procedures.
Biochemical terbium phospho-specific activity
Label or Dye
FRAP1 (mTOR), FRAP1 (mTOR)
Contents & Storage
GFP-4E-BP1 protein substrate in 10 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol, 2mM DTT. Store at -80°C.
Figure 1 - LanthaScreen™ kinase assay using physiological protein substrates
Figure 2 - Representative data for GFP-ATF2 (19-96) as a substrate for JNK1 kinase assays
Figure 4 - Representative data for GFP-4E-BP1 as a substrate for FRAP1 (mTOR) assay
Table 1 - Reactivity table for physiological protein substrates
Figure 3 - Representative data for GFP-c-Jun (1-179) as a substrate for JNK1 kinase assays