This product will be discontinued soon. Its replacement is the SiteClick™ Qdot® 605 Antibody Labeling Kit.
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Qdot® 605 Antibody Conjugation Kit

Catalog number: Q22001MP

Molecular Probes™  Related applications: Cellular Imaging | Labeling Chemistry | Protein Labeling & Crosslinking

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Qdot® Antibody Conjugation Kits provide a method to conjugate your antibodies quantum dot nanocrystals. The kit contains amine-derivatized, PEG-coated nanocrystals and the amine-thiol crosslinker, SMCC. In addition to antibodies, other thiol-containing molecules can be coupled to these Qdot® nanocrystals using this kit. Primary antibodies labeled using these methods are excellent reagents in flow cytometry, fluorescence imaging, western blots, and more. The kits are available in eight Qdot® nanocrystal colors and supply the reagents needed for two labeling and separation reactions.

Important Features of Qdot® Antibody Conjugation Kits
• Qdot® 605 quantum dot has emission maxima of ~605 nm
• Labeled proteins typically ready to use in 4–5 hours
• Optimized for ~300 μg of whole IgG or polyclonal Fab fragments
• Requires the antibody or protein be purified and reduced prior to labeling

Properties of Qdot® Nanocrystals
The Qdot® nanocrystal is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of detection reagents. Qdot® nanocrystals are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 nanocrystals, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Qdot® Nanocrystals Are Our Brightest Labels

• Extremely photostable and bright fluorescence (very high fluorescence quantum yield)
• Efficiently excited with single-line excitation sources
• Narrow emission profiles, large Stokes shift

Qdot® nanocrystals are our brightest fluorescent option for labeling, with molar extinction coefficients several orders of magnitude brighter than our brightest organic dyes. Qdot® nanocrystals are an excellent choice for primary antibody based detection or for rare event and even single-molecule studies. By avoiding secondary detection, primary antibody–based approaches compress workflows in addition to allowing matched isotype/species antibodies to be used.

Find Out More About Qdot® Nanocrystals
Read more about Qdot® nanocrystals, review additional product details in Qdot® Nanocrystals—Section 6.6 in the Molecular Probes® Handbook, and learn about protocol modifications that may enhance coupling using our Qdot® Antibody Conjugation Kits.

Learn More About Antibody and Protein Labeling
We offer a wide selection of Molecular Probes® antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes® Handbook.

We’ll Make a Custom Antibody Conjugate for You
If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


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Color: Red
Label or Dye: Qdot® 605
Product Size: 1 kit
Emission Class: Visible
Labeling Scale: 300 µg
Labeling Target: Antibodies (General)
Detection Method: Fluorescent
Shipping Condition: Room Temperature
Excitation⁄Emission (nm): 405,488⁄625

Contents & storage

Store in refrigerator (2–8°C).


Manuals & protocols

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