Invitrogen™

Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Catalog number:  T1011

 Related applications:

Protein Assays and Analysis

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Description

The Galacto-Light Plus™ Beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of β-galactosidase in reporter gene assays.

• The Galacto-Light Plus™ Beta-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Ready-to-Use
The Galacto-Light™ Plus™ system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.

Products described herein are guaranteed for 12 months from the date of purchase.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: β-Gal (lacZ) Assay
Compatible Cells: Mammalian Cells
Detection Method: Chemiluminescent
For Use With (Equipment): Luminometer (Microplate)
Form: Liquid
Format: 96-well plate
High Throughput Compatibility: High Throughput-Compatible
Product Line: NovaBright™
Product Size: 600 assays
Readout: End Point
Sample Type (Specific): Cell Cultures
Substrate: Galacton-Plus®
Substrate Properties: Chemical Substrate
Substrate Type: Beta-Gal Substrate
Target Enzyme: Beta-Galactosidase
Technique: Enhanced Chemiluminescence

Contents & storage

This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C.

Documents

Manuals & protocols