pPICZα A, B, & C Pichia Vectors

Catalog number: V19520

Invitrogen™  Related applications: Protein Expression

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Description

pPICα A, B, and C vectors are 3.6 kb vectors used to express and secrete recombinant proteins in Pichia pastoris. Recombinant proteins are expressed as fusions to an N-terminal peptide encoding the Saccharomyces cerevisiae á-factor secretion signal. These vectors allow high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in any Pichia strain including X-33, SMD1168H, and KM71H. pPICα vectors contain the following elements:


• Contains AOX1 promoter for tightly regulated, methanol-induced expression of the gene of interest
• All three reading frames (A, B, C versions) are provided to facilitate in-frame cloning with the C-terminal peptide
• α-factor secretion signal for directing secreted expression of the recombinant protein
• Zeocin resistance gene for selection in both E. coli and Pichia
• C-terminal peptide containing the c-myc epitope and a polyhistidine (6xHis) tag for detection and purification of a recombinant fusion protein
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance (Bacterial): Zeocin™ (ZeoR)
Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 20 µg
Promoter: AOX1
Protein Tag or Fusion: His Tag (6x), c-Myc Epitope Tag
Vector Type: pPIC
Shipping Condition: Room Temperature

Contents & storage

20 µg each of lyophilized pPICZ A, B, and C is supplied (Store at -20C)

Documents

Manuals & protocols

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