pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection, high-level expression, and rapid protein purification and detection. The pPIC6 A, B, & C vectors are designed for high-level secreted expression. Both sets of vectors have the following features: Blasticidin resistance for direct selection of multi-copy integrants Inducible AOX1 promoter for high-level expression in Pichia pastoris C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody pPIC6 also features: An É -factor secretion signal for efficient transport of proteins to the medium
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
AOX1
Product Type
Pichia Expression Vector
Protein Tag
His Tag (6x)
Contents & Storage
Each vector kit includes 20 μg each of expression vector in A, B, & C versions, 20 μg of a lacZ (intracellular) or HSA (secreted) control vector, and an X-33 Pichia stab. The Starter Kits also include 50 mg of Blasticidin. Store vectors and Blasticidin at -20°C. Store the Pichia stab at +4°C. All reagents are guaranteed stable for 6 months when properly stored.