Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry
Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry
Invitrogen™

Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains:Read more
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Catalog number V23201
Price (USD)/ 1 kit
393.00
-
Add to cart
Price (USD)/ 1 kit
393.00
Add to cart
Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO™-1, and PI dyes, for flow cytometry
Catalog numberV23201
Price (USD)/ 1 kit
393.00
-
Add to cart

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide. The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot. Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence. Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells. The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity1 kit
FormatKit
Product TypeDead Cell Apoptosis Kit
Flow Cytometer Laser Lines488 nm
Excitation/EmissionYO-PRO™-1: 491/509 cm, Hoechst 33342: 350/461 cm, PI: 535/617 cm
No. of Reactions200 Reactions
Shipping ConditionRoom Temperature
Product LineVybrant™, YO-PRO™
For Use With (Application)Flow Cytometry
ConjugateHoechst 33342, Propidium Iodide, YO-PRO-1
For Use With (Equipment)Flow Cytometer
FormSolution
Unit Size1 kit
Contents & Storage
Contains 1 vial of Hoechst 33342, 1 vial of YO-PRO™-1, and propidium iodide.

Store in refrigerator (2–8°C) and protect from light.