Invitrogen™

Vivid Colors™ pcDNA™6.2/C-YFP-DEST Vector

Back to top

Description

The Vivid Colors™ Fluorescent Protein Gateway® Destination Vectors allow you to quickly and easily fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pcDNA™ 6.2 Gateway™ mammalian expression vector. These powerful Gateway® Technology vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), or the popular Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein. Both fluorescent proteins have been humanized for optimal mammalian expression (3). In addition, the Vivid Colors™ pcDNA™ 6.2 Fluorescent Protein Gateway® Vectors offer:

• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Options to fuse EmGFP or YFP to the N- and C-terminus of your protein
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Key Functions: Reporter Assays, Subcellular Localization
Product Line: Gateway®, Vivid Colors™, pcDNA™
Cloning Method: Gateway®
Protein Tag: YFP
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
Promoter: CMV
Reporter Gene: YFP
Selection Agent (Eukaryotic): Blasticidin
Vector: pDEST, pcDNA

Contents & storage

Each vector is supplied as supercoiled and lyophilized plasmid DNA. Store at -20°C after resuspended. Guaranteed stable for 6 months when properly stored.