The pBAD/Myc-His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The pBAD/Myc-His vector expresses native proteins or fusion proteins with a C-terminal tag. The vector provides:
• The araBAD promoter for tightly regulated expression • Translation initiation signals optimized for E. coliexpression • C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin or detection with an Anti-His(C-term) Antibody • C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody
Three vectors are provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
For Research Use Only. Not for use in diagnostic procedures.
Constitutive or Inducible System
Bacterial Expression Vector
Selection Agent (Eukaryotic)
Antibiotic Resistance Bacterial
EK (Enterokinase) Recognition Site
c-Myc Epitope Tag
Contents & Storage
The pBAD/Myc-His Kit includes 20 µg of pBAD/Myc-His A, B, & C vector, 20 µg of pBAD/Myc-His/lacZ, 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.