NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Midi Protein Gel, 26-well

Catalog number:  WG1603BOX

Related applications:

Protein Electrophoresis & Western Blotting

Back to top


Invitrogen NuPAGE Tris-Acetate protein gels provide excellent separation of large molecular weight proteins. They are high performance polyacrylamide gels that simulate the denaturing or native conditions of the traditional laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of high molecular weight proteins compared to traditional Tris-glycine SDS-PAGE gels.

Features of NuPAGE Tris-Acetate gels:
High resolution—gels offer optimal separation of high molecular weight proteins
Better protein integrity—sample preparation process has been optimized to help preserve proteins
Longer self life—gels can be stored for at least eight months

Learn more about our NuPAGE Tris-Acetate gels ›
View migration charts ›

Choose the right NuPAGE Tris-Acetate gel for your protein separation
Obtain optimal separation of your high molecular weight proteins by choosing the right combination of gel and running buffer. NuPAGE Tris-Acetate protein gels come in a polyacrylamide concentration of 7% and a 3–8% gradient. Gels come in two sizes: mini (8 cm x 8 cm) or midi (8.7 cm x 13.3 cm) and either 1.0 mm (mini and midi gels) or 1.5 mm (mini gel format only) in thickness. NuPAGE Tris-Acetate gels also come in multiple well formats. Mini gels can be run using our XCell SureLock Mini-Cell or Mini Gel Tank. Midi gels can be run using our XCell4 SureLock Midi-Cell or conveniently with the Bio-Rad Criterion™ Cell using our adapters.

Run your proteins in native or denatured form
NuPAGE Tris-Acetate protein gels do not contain SDS and can be used to separate proteins in native or denatured form. For denatured proteins, we recommend using NuPAGE LDS Sample Buffer and NuPAGE Tris-Acetate SDS Running Buffer. For native proteins, we recommend using Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer for traditional wet transfer using the XCell II Blot Module or the Mini Blot Module. Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device.

Related links
Overview of 1D Protein Electrophoresis

For Research Use Only. Not for use in diagnostic procedures.


Adapters: No Adapters
Mode of Separation: Molecular Weight
Product Line: Novex™, NuPAGE™
Shipping Condition: Wet Ice
System Type: NuPAGE™ Novex™
Thickness: 1.0 mm
Well Design: 1D Well
Product Type Specs: Gradient Page
Recommended Applications: Denaturing, Native
Sample Loading Volume: up to 15 µL
Length (Metric): 8 cm
Length (Metric) Gel: 13 cm
Width (Metric): 8 cm
Width (Metric) Gel: 8 cm
Gel Size: Midi
Separation Range: 36 to 500 kd
Gel Percentage: 3 to 8%
Gel Type: Tris-Acetate
Separation Type: Denaturing, Native
Gel Thickness: 1.0 mm
For Use With (Equipment): XCell4 SureLock Midi
Separation of: Protein
Wells: 26-well
Quantity: 1 Box

Contents & storage

One box contains 10 gels. Store in refrigerator (2–8°C). Do not freeze. Shelf life is 6 months.