The ZOOM™ IPGRunner™ system is the first system to offer oil-free, 2D electrophoresis in a mini-gel format. First dimension separation isoelectric focusing (IEF) is typically complete in less than three hours. The mini-gel design of the system is easy to handle, eliminates mineral oil overlays, and allows processing of up to 12 samples at once for high-throughput usage.
The ZOOM™ IPGRunner™ Combo Kit includes everything needed for first dimension separation:
• ZOOM™ IPGRunner™ Mini-Cell
• 10 ZOOM™ IPGRunner™ Cassettes
• 12 ZOOM™ Strips, pH 3-10NL
For second dimension separation, Novex™ ZOOM™ gels and the
XCell SureLock™ Mini-Cell are recommended (both are available separately).
ZOOM™ IPGRunner™ Mini-Cell The ZOOM™ IPGRunner™ Mini-Cell consists of a Zoom™ IPGRunner Core, a mini-cell chamber, and a lid. The ZOOM™ IPGRunner™ Core can be used to perform isoelectric focusing of up to 12 IPG strips (ZOOM™ Strips) loaded in two ZOOM™ IPGRunner™ Cassettes.
ZOOM™ IPGRunner™ Cassette The ZOOM™ IPGRunner™ Cassette is used to perform oil-free rehydration and isoelectric focusing of up to six 7.0 cm IPG strips (ZOOM™ Strips) without requiring separate units for rehydration and isoelectric focusing. The ZOOM™ IPGRunner™ Cassettes are disposable.
ZOOM™ Strips ZOOM™ Strips are 7 cm long and contain a thin layer of polyacrylamide gel with a fixed pH gradient. Each ZOOM™ Strip is clearly labeled with a unique identifying number, pH range, and orientation marks (Figure 3). ZOOM™ Strips are supplied attached to a tri-fold card (Figure 4) for easy access and removal.
Novex™ ZOOM™ Gels (available separately) ZOOM™ Gels are pre-cast polyacrylamide gels designed for 2D analysis of proteins following isoelectric focusing with ZOOM™ Strips. They are recommended for use with our XCell SureLock™ Mini-Cell. ZOOM™ Gels contain an IPG well and a molecular weight marker well. The IPG well is designed to accommodate the 7.0 cm ZOOM™ Strips. Two types of ZOOM™ Gels are available separately:
NuPAGE™ Novex™ 4–12% Bis-Tris ZOOM™ GelNovex™ 4–20% Tris-Glycine ZOOM™ GelFor Research Use Only. Not for use in diagnostic procedures.