Seminar
Jul 04, 2017 - Jul 04, 2017
Go beyond with proven proteomics solutions

Join us at the 5th International Congress on Analytical Proteomics | 3-6 July, Costa de Caparica, Portugal

Meet our scientists and representatives at booth #2 to learn more and discuss how we can partner with you to identify proteins faster and more accurately, so you can go beyond the limit of what was ever thought possible.

Register today to attend our lunch seminar on Tuesday, July 4th | 01:00 – 02:00 PM

Unmatched Confidence for Quantitative and Structural Proteomics Analyses with Orbitrap Technology

Hotel Aldeia dos Capuchos Golf & SPA, Room A -  | Largo Aldeia dos Capuchos, 2825-017 Caparica, Portuga
A Promising Alternative to MS2-DIA: IonStar on an Ultra-High-Field Orbitrap Enables Large-scale, Accurate and Extensive Proteomics Quantification with Low Missing Data and False Positives
A Promising Alternative to MS2-DIA: IonStar on an Ultra-High-Field Orbitrap Enables Large-scale, Accurate and Extensive Proteomics Quantification with Low Missing Data and False Positives

In-depth and reproducible protein measurement especially with many biological/individual replicates is critical for pharmaceutical/clinical proteomics, but remains highly challenging owing to suboptimal accuracy, reproducibility and false-positive-biomarker-discovery, and high levels of missing data. MS2-based Data-Independent-Acquisition(MS2-DIA) approaches were developed to lower missing values but often with limited depth. To address these challenges, we developed an “IonStar” workflow consisting of experimental components based on the use of an Ultra-high-field Orbitrap  and a data processing pipeline to take full advantage of high-resolution MS1 ion-current-based quantification, which achieves accurate, precise and in-depth protein quantification with low-missing-values in large cohorts. This pipeline was thoroughly compared to popular methods including spectral counting, OpenMS and Maxquant, using a 5-group, 20-replicates benchmark sampleset. IonStar showed by far the most in-depth analysis, best accuracy, lowest intra-group-variation(~5%CV vs.11-18% by others), missing data(<0.2% vs.18-44%) and false-positive-biomarker-discovery-rate(<4% vs.7-31%), with quantified protein abundances spanning ~5.8 orders of magnitudes(vs.3.2-4.4). As applications, we quantified >6000 protein groups, >99% without missing data, in large biological cohorts (50-100 samples).

Speaker: Jun Qu, University at Buffalo, Buffalo, NY, USA

Novel Data Acquisition and Analysis Strategies for High Confident Cross-link Identification Using Orbitrap Mass Spectrometers
Novel Data Acquisition and Analysis Strategies for High Confident Cross-link Identification Using Orbitrap Mass Spectrometers

Chemical cross-linking combined with mass spectrometry (CX-MS) is a powerful method to probe the structure of proteins, complexes and interactions. The high resolution and mass accuracy of Orbitrap technology, in combination with enhanced fragmentation modes (e.g., HCD, ETD, EtHCD) and MSn modalities, allow completely unique and effective methods to probe protein structure. New reagents and software facilitate sample preparation and data interpretation to make these capabilities available to all labs.

Speaker: Sega Ndiaye, Thermo Fisher Scientific, France

Lunch will be provided. Event is free but registration is mandatory.